Transcriptomic analyses in a benthic fish exposed to contaminated estuarine sediments through laboratory and in situ bioassays

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorCosta, Pedro M.-
Autor(es): dc.creatorMiguel, Célia-
Autor(es): dc.creatorCaeiro, Sandra-
Autor(es): dc.creatorLobo, Jorge-
Autor(es): dc.creatorMartins, Marta-
Autor(es): dc.creatorFerreira, Ana.M.-
Autor(es): dc.creatorCaetano, Miguel-
Data de aceite: dc.date.accessioned2022-02-15T14:09:53Z-
Data de disponibilização: dc.date.available2022-02-15T14:09:53Z-
Data de envio: dc.date.issued2021-09-29-
Data de envio: dc.date.issued2021-09-29-
Data de envio: dc.date.issued2011-
Data de envio: dc.date.issued2021-09-20-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/10400.2/11147-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/10400.2/11147-
Descrição: dc.descriptionThe transcription of contaminant responserelated genes was investigated in juvenile Senegalese soles exposed to sediments from three distinct sites (a reference plus two contaminated) of a Portuguese estuary (the Sado, W Portugal) through simultaneous 28-day laboratory and in situ bioassays. Transcription of cytochrome P450 1A (CYP1A), metallothionein 1 (MT1), glutathione peroxidase (GPx), catalase (CAT), caspase 3 (CASP3) and 90 kDa heat-shock protein alpha (HSP90AA) was surveyed in the liver by real-time PCR. CASP3 transcription analysis was complemented by surveying apoptosis through the TUNEL reaction. After 14 days of exposure, relative transcription was either reduced or decreased in fish exposed to the contaminated sediments, revealing a disturbance stress phase during which animals failed to respond to insult. After 28 days of exposure all genes’ transcription responded to contamination but laboratory and in situ assays depicted distinct patterns of regulation. Although sediments revealed a combination of organic and inorganic toxicants, transcription of the CYP1A gene was consistently correlated to organic contaminants. Metallothionein regulation was found correlated to metallic and organic xenobiotic contamination in the laboratory and in situ, respectively. The transcription of oxidative stress-related genes can be a good indicator of general stress but caution is mandatory when interpreting the results since regulation may be influenced by multiple factors. As for MT1, HSP90 up-regulation has potential to be a good indicator for total contamination, as well as the CASP3 gene, even though hepatocyte apoptosis depicted values inconsistent with sediment contamination, showing that programmed cell death did not directly depend on caspase transcription alone.-
Descrição: dc.descriptioninfo:eu-repo/semantics/publishedVersion-
Idioma: dc.languageen-
Relação: dc.relationPOCTI/AMB 57281/104-
Relação: dc.relationinfo:eu-repo/grantAgreement/FCT/POSI/19815/PT-
Direitos: dc.rightsopenAccess-
Palavras-chave: dc.subjectToxicogenomics-
Palavras-chave: dc.subjectTranscriptomics-
Palavras-chave: dc.subjectSolea senegalensis-
Palavras-chave: dc.subjectQuantitative real-time RT-PCR-
Palavras-chave: dc.subjectApoptosis-
Palavras-chave: dc.subjectEcological risk assessment-
Palavras-chave: dc.subjectODS::14:Proteger a Vida Marinha-
Título: dc.titleTranscriptomic analyses in a benthic fish exposed to contaminated estuarine sediments through laboratory and in situ bioassays-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Aberto - Universidade Aberta (Portugal)

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