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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.creator | Herrera, Christiane Ribeiro Janner | - |
Autor(es): dc.creator | Vieira, Vanessa Rodrigues | - |
Autor(es): dc.creator | Benoliel, Tiago | - |
Autor(es): dc.creator | Carneiro, Clara Vida Galvão Corrêa | - |
Autor(es): dc.creator | De Marco, Janice Lisboa | - |
Autor(es): dc.creator | Moraes, Lídia Maria Pepe de | - |
Autor(es): dc.creator | Almeida, João Ricardo Moreira de | - |
Autor(es): dc.creator | Torres, Fernando Araripe Gonçalves | - |
Data de aceite: dc.date.accessioned | 2024-10-23T16:41:17Z | - |
Data de disponibilização: dc.date.available | 2024-10-23T16:41:17Z | - |
Data de envio: dc.date.issued | 2021-07-26 | - |
Data de envio: dc.date.issued | 2021-07-26 | - |
Data de envio: dc.date.issued | 2020 | - |
Fonte completa do material: dc.identifier | https://repositorio.unb.br/handle/10482/41492 | - |
Fonte completa do material: dc.identifier | https://doi.org/10.3390/microorganisms9071372 | - |
Fonte completa do material: dc.identifier | https://orcid.org/0000-0002-5307-9168 | - |
Fonte completa do material: dc.identifier | https://orcid.org/0000-0002-2902-5805 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/capes/914717 | - |
Descrição: dc.description | Sugarcane bagasse is an agricultural residue rich in xylose, which may be used as a feedstock for the production of high-value-added chemicals, such as xylonic acid, an organic acid listed as one of the top 30 value-added chemicals on a NREL report. Here, Zymomonas mobilis was engineered for the first time to produce xylonic acid from sugarcane bagasse hydrolysate. Seven coding genes for xylose dehydrogenase (XDH) were tested. The expression of XDH gene from Paraburkholderia xenovorans allowed the highest production of xylonic acid (26.17 ± 0.58 g L−1) from 50 g L−1 xylose in shake flasks, with a productivity of 1.85 ± 0.06 g L−1 h −1 and a yield of 1.04 ± 0.04 gAX/gX. Deletion of the xylose reductase gene further increased the production of xylonic acid to 56.44 ± 1.93 g L−1 from 54.27 ± 0.26 g L−1 xylose in a bioreactor. Strain performance was also evaluated in sugarcane bagasse hydrolysate as a cheap feedstock, which resulted in the production of 11.13 g L−1 xylonic acid from 10 g L−1 xylose. The results show that Z. mobilis may be regarded as a potential platform for the production of organic acids from cheap lignocellulosic biomass in the context of biorefineries. | - |
Formato: dc.format | application/pdf | - |
Publicador: dc.publisher | MDPI | - |
Direitos: dc.rights | Acesso Aberto | - |
Direitos: dc.rights | Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | - |
Palavras-chave: dc.subject | Xilose | - |
Palavras-chave: dc.subject | Biomassa lignocelulósica | - |
Palavras-chave: dc.subject | Ácido xilônico | - |
Palavras-chave: dc.subject | Zymomonas mobilis | - |
Título: dc.title | Engineering Zymomonas mobilis for the production of xylonic acid from sugarcane bagasse hydrolysate | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional – UNB |
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