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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.creator | Parente, Juliana Alves | - |
Autor(es): dc.creator | Salem-Izacc, Sílvia Maria | - |
Autor(es): dc.creator | Santana, Jaime Martins de | - |
Autor(es): dc.creator | Pereira, Maristela | - |
Autor(es): dc.creator | Borges, Clayton Luiz | - |
Autor(es): dc.creator | Bailão, Alexandre Melo | - |
Autor(es): dc.creator | Soares, Célia Maria de Almeida | - |
Data de aceite: dc.date.accessioned | 2024-10-23T16:39:21Z | - |
Data de disponibilização: dc.date.available | 2024-10-23T16:39:21Z | - |
Data de envio: dc.date.issued | 2013-01-29 | - |
Data de envio: dc.date.issued | 2013-01-29 | - |
Data de envio: dc.date.issued | 2010-09 | - |
Fonte completa do material: dc.identifier | http://repositorio.unb.br/handle/10482/11978 | - |
Fonte completa do material: dc.identifier | https://dx.doi.org/10.1186/1471-2180-10-292 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/capes/913916 | - |
Descrição: dc.description | Background Paracoccidioides brasiliensis is a thermodimorphic fungus, the causative agent of paracoccidioidomycosis (PCM). Serine proteases are widely distributed and this class of peptidase has been related to pathogenesis and nitrogen starvation in pathogenic fungi. Results A cDNA (Pbsp) encoding a secreted serine protease (PbSP), was isolated from a cDNA library constructed with RNAs of fungal yeast cells recovered from liver of infected mice. Recombinant PbSP was produced in Escherichia coli, and used to develop polyclonal antibodies that were able to detect a 66 kDa protein in the P. brasiliensis proteome. In vitro deglycosylation assays with endoglycosidase H demonstrated that PbSP is a N-glycosylated molecule. The Pbsp transcript and the protein were induced during nitrogen starvation. The Pbsp transcript was also induced in yeast cells infecting murine macrophages. Interactions of PbSP with P. brasiliensis proteins were evaluated by two-hybrid assay in the yeast Saccharomyces cerevisiae. PbSP interacts with a peptidyl prolyl cis-trans isomerase, calnexin, HSP70 and a cell wall protein PWP2. Conclusions A secreted subtilisin induced during nitrogen starvation was characterized indicating the possible role of this protein in the nitrogen acquisition. PbSP interactions with other P. brasiliensis proteins were reported. Proteins interacting with PbSP are related to folding process, protein trafficking and cytoskeleton reorganization. | - |
Formato: dc.format | application/pdf | - |
Publicador: dc.publisher | BioMed Central | - |
Direitos: dc.rights | Acesso Aberto | - |
Direitos: dc.rights | ©2010 Parente et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | - |
Palavras-chave: dc.subject | Paracoccidioides brasiliensis | - |
Palavras-chave: dc.subject | Paracoccidioidomicose | - |
Palavras-chave: dc.subject | Fungos | - |
Título: dc.title | A secreted serine protease of Paracoccidioides brasiliensis and its interactions with fungal proteins | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional – UNB |
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