L-Asparaginase type II from fusarium proliferatum : heterologous expression and in silico analysis

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Brasilia, Health Science School-
Autor(es): dc.contributorUniversity of Brasilia, Health Science School-
Autor(es): dc.contributorBrazilian Agricultural Research Corporation—EMBRAPA Agroenergia-
Autor(es): dc.contributorUniversity of Brasilia, Health Science School-
Autor(es): dc.contributorUniversity of Brasilia, Institute of Biological Sciences-
Autor(es): dc.contributorBrazilian Agricultural Research Corporation—EMBRAPA Agroenergia-
Autor(es): dc.contributorUniversity of Brasilia, Health Science School-
Autor(es): dc.contributorUniversity of São Paulo, Department of Biochemical and Pharmaceutical Technology-
Autor(es): dc.contributorUniversity of Brasilia, Health Science School-
Autor(es): dc.contributorUniversity of Brasilia, Health Science School-
Autor(es): dc.contributorUniversity of Brasilia, Health Science School-
Autor(es): dc.contributorUniversity of Brasilia, Health Science School-
Autor(es): dc.creatorCardoso, Samuel Leite-
Autor(es): dc.creatorSouza, Paula Monteiro de-
Autor(es): dc.creatorRodrigues, Kelly Barreto-
Autor(es): dc.creatorMota, Isabella de Souza-
Autor(es): dc.creatorFerreira Filho, Edivaldo Ximenes-
Autor(es): dc.creatorFávaro, Léia Cecilia de Lima-
Autor(es): dc.creatorAraujo, Felipe Saldanha de-
Autor(es): dc.creatorHomem de Mello, Mauricio-
Autor(es): dc.creatorPessoa, Adalberto-
Autor(es): dc.creatorDuarte, Dâmaris Silveira-
Autor(es): dc.creatorBazzo, Yris Maria Fonseca-
Autor(es): dc.creatorBatista, Pérola de Oliveira Magalhães Dias-
Data de aceite: dc.date.accessioned2024-10-23T16:26:27Z-
Data de disponibilização: dc.date.available2024-10-23T16:26:27Z-
Data de envio: dc.date.issued2024-09-20-
Data de envio: dc.date.issued2024-09-20-
Data de envio: dc.date.issued2023-09-20-
Fonte completa do material: dc.identifierhttp://repositorio.unb.br/handle/10482/50395-
Fonte completa do material: dc.identifierhttps://doi.org/10.3390/pharmaceutics15092352-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0003-1824-2211-
Fonte completa do material: dc.identifierhttps://orcid.org/0009-0002-9014-3488-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0003-4947-8753-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0003-3260-0310-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-4541-9177-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0003-3260-0310-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-4541-9177-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-5268-8690-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0003-1851-5224-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/908532-
Descrição: dc.descriptionThe search for new drug-producing microorganisms is one of the most promising situations in current world scientific scenarios. The use of molecular biology as well as the cloning of protein and compound genes is already well established as the gold standard method of increasing productivity. Aiming at this increase in productivity, this work aims at the cloning, purification and in silico analysis of l-asparaginase from Fusarium proliferatum in Komagataella phaffii (Pichia pastoris) protein expression systems. The l-asparaginase gene (NCBI OQ439985) has been cloned into Pichia pastoris strains. Enzyme production was analyzed via the quantification of aspartic B-hydroxamate, followed by purification on a DEAE FF ion exchange column. The in silico analysis was proposed based on the combined use of various technological tools. The enzymatic activity found intracellularly was 2.84 IU/g. A purification factor of 1.18 was observed. The in silico analysis revealed the position of five important amino acid residues for enzymatic activity, and likewise, it was possible to predict a monomeric structure with a C-score of 1.59. The production of the enzyme l-asparaginase from F. proliferatum in P. pastoris was demonstrated in this work, being of great importance for the analysis of new methodologies in search of the production of important drugs in therapy.-
Descrição: dc.descriptionFaculdade de Ciências da Saúde (FS)-
Descrição: dc.descriptionInstituto de Ciências Biológicas (IB)-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Publicador: dc.publisherMDPI-
Direitos: dc.rightsAcesso Aberto-
Direitos: dc.rightsCopyright: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).-
Palavras-chave: dc.subjectL -asparaginase-
Palavras-chave: dc.subjectSilos graneleiros - análise-
Palavras-chave: dc.subjectFungos fitopatogênicos-
Título: dc.titleL-Asparaginase type II from fusarium proliferatum : heterologous expression and in silico analysis-
Aparece nas coleções:Repositório Institucional – UNB

Não existem arquivos associados a este item.