Atenção: Todas as denúncias são sigilosas e sua identidade será preservada.
Os campos nome e e-mail são de preenchimento opcional
Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.creator | Almeida, Simoneide Souza Titze de | - |
Autor(es): dc.creator | Horst, Camila Hillesheim | - |
Autor(es): dc.creator | Sánchez, Cristina Soto | - |
Autor(es): dc.creator | Fernandez, Eduardo | - |
Autor(es): dc.creator | Almeida, Ricardo Titze de | - |
Data de aceite: dc.date.accessioned | 2024-10-23T16:09:10Z | - |
Data de disponibilização: dc.date.available | 2024-10-23T16:09:10Z | - |
Data de envio: dc.date.issued | 2020-11-23 | - |
Data de envio: dc.date.issued | 2020-11-23 | - |
Data de envio: dc.date.issued | 2018-07-23 | - |
Fonte completa do material: dc.identifier | https://repositorio.unb.br/handle/10482/39653 | - |
Fonte completa do material: dc.identifier | https://doi.org/10.3390/molecules23071825 | - |
Fonte completa do material: dc.identifier | https://orcid.org/0000-0001-7286-1676 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/capes/901092 | - |
Descrição: dc.description | MicroRNAs (miRNAs) regulate gene expression at posttranscriptional level by triggering RNA interference. In such a sense, aberrant expressions of miRNAs play critical roles in the pathogenesis of many disorders, including Parkinson’s disease (PD). Controlling the level of specific miRNAs in the brain is thus a promising therapeutic strategy for neuroprotection. A fundamental need for miRNA regulation (either replacing or inhibition) is a carrier capable of delivering oligonucleotides into brain cells. This study aimed to examine a polymeric magnetic particle, Neuromag®, for delivery of synthetic miRNA inhibitors in the rat central nervous system. We injected the miRNA inhibitor complexed with Neuromag® into the lateral ventricles next to the striatum, by stereotaxic surgery. Neuromag efficiently delivered oligonucleotides in the striatum and septum areas, as shown by microscopy imaging of fluorescein isothiocyanate (FITC)-labeled oligos in astrocytes and neurons. Transfected oligos showed efficacy concerning miRNA inhibition. Neuromag®-structured miR-134 antimiR (0.36 nmol) caused a significant 0.35 fold decrease of striatal miR-134, as revealed by real-time quantitative polymerase chain reaction (RT-qPCR). In conclusion, the polymeric magnetic particle Neuromag® efficiently delivered functional miRNA inhibitors in brain regions surrounding lateral ventricles, particularly the striatum. This delivery system holds potential as a promising miRNA-based disease-modifying drug and merits further pre-clinical studies using animal models of PD. | - |
Descrição: dc.description | Faculdade de Agronomia e Medicina Veterinária (FAV) | - |
Formato: dc.format | application/pdf | - |
Publicador: dc.publisher | MDPI | - |
Direitos: dc.rights | Acesso Aberto | - |
Direitos: dc.rights | © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | - |
Palavras-chave: dc.subject | MicroRNA | - |
Palavras-chave: dc.subject | Nanopartículas | - |
Palavras-chave: dc.subject | Neuromag® | - |
Título: dc.title | Delivery of miRNA-targeted oligonucleotides in the rat striatum by magnetofection with Neuromag® | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional – UNB |
O Portal eduCAPES é oferecido ao usuário, condicionado à aceitação dos termos, condições e avisos contidos aqui e sem modificações. A CAPES poderá modificar o conteúdo ou formato deste site ou acabar com a sua operação ou suas ferramentas a seu critério único e sem aviso prévio. Ao acessar este portal, você, usuário pessoa física ou jurídica, se declara compreender e aceitar as condições aqui estabelecidas, da seguinte forma: