Cryopreservation and characterization of canine preantral follicles

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorRôlo, José Luiz Jivago de Paula-
Autor(es): dc.creatorPaulini, F.-
Autor(es): dc.creatorSilva, R. C.-
Autor(es): dc.creatorAraujo, M. S.-
Autor(es): dc.creatorMarinho, A. P. S.-
Autor(es): dc.creatorLucci, C. M.-
Data de aceite: dc.date.accessioned2024-10-23T15:49:58Z-
Data de disponibilização: dc.date.available2024-10-23T15:49:58Z-
Data de envio: dc.date.issued2019-01-22-
Data de envio: dc.date.issued2019-01-22-
Data de envio: dc.date.issued2018-
Fonte completa do material: dc.identifierhttp://repositorio.unb.br/handle/10482/33799-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/893142-
Descrição: dc.descriptionThe aim of this study was to define the population, morphological and ultrastructural characteristics of bitch preantral follicles (PAFs) and to compare the effects on the morphology of PAF of two cryopreservation techniques - slow freezing (SF) and vitrification (V) - of bitches' ovarian tissue. The average population (number per ovary) of PAFs was 48,541 ± 18,366, where 94.25% were primordial (45,145 ± 16,076). The average diameter of the primordial follicles was 27.5 ± 4.2 μm. The overall percentage of morphologically normal PAFs was 93.66 ± 6.81% for the control group, 86.16 ± 11.05% after SF and 68.14 ± 12.75% after V. The percentage of normal primordial follicles was 96.69 ± 4.72% in control, 89.51 ± 10.39% in SF and 75.32 ± 9.23% in V. There was no significant difference in the overall percentage of normal PAFs among SF and the control. However, slow frozen follicles presented ultrastructural damage, while vitrified primordial and primary follicles were well preserved. In conclusion, although slow freezing seemed to be a good preserving method, vitrification was more effective than slow freezing in preserving the ultrastructure of primordial and primary follicles of bitches.-
Formato: dc.formatapplication/pdf-
Publicador: dc.publisherElsevier-
Direitos: dc.rightsAcesso Aberto-
Direitos: dc.rightsAutorização concedida ao Repositório Institucional da Universidade de Brasília (RIUnB) pelo servidor José Luiz Jivago de Paula Rôlo, em 15 de janeiro de 2019, para disponibilizar o artigo, gratuitamente, para fins de leitura, impressão e/ou download, a título de divulgação da obra.-
Palavras-chave: dc.subjectCriopreservação de órgãos, tecidos, etc.-
Palavras-chave: dc.subjectCão - reprodução-
Título: dc.titleCryopreservation and characterization of canine preantral follicles-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional – UNB

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