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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.creator | Nery, Flávia M. B. | - |
Autor(es): dc.creator | Melo, Fernando Lucas | - |
Autor(es): dc.creator | Boiteux, Leonardo Silva | - |
Autor(es): dc.creator | Ribeiro, Simone G. | - |
Autor(es): dc.creator | Resende, Renato de Oliveira | - |
Autor(es): dc.creator | Orílio, Anelise Franco | - |
Autor(es): dc.creator | Batista, Josiane Goulart | - |
Autor(es): dc.creator | Lima, Mirtes Freitas | - |
Autor(es): dc.creator | Carvalho, Rita de Cássia Pereira | - |
Data de aceite: dc.date.accessioned | 2024-10-23T15:47:05Z | - |
Data de disponibilização: dc.date.available | 2024-10-23T15:47:05Z | - |
Data de envio: dc.date.issued | 2020-10-21 | - |
Data de envio: dc.date.issued | 2020-10-21 | - |
Data de envio: dc.date.issued | 2020-08-27 | - |
Fonte completa do material: dc.identifier | https://repositorio.unb.br/handle/10482/39576 | - |
Fonte completa do material: dc.identifier | https://doi.org/10.3390/v12090950 | - |
Fonte completa do material: dc.identifier | https://orcid.org/0000-0002-5791-8002 | - |
Fonte completa do material: dc.identifier | https://orcid.org/0000-0002-4965-1424 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/capes/891904 | - |
Descrição: dc.description | In a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered. | - |
Formato: dc.format | application/pdf | - |
Publicador: dc.publisher | MDPI | - |
Direitos: dc.rights | Acesso Aberto | - |
Direitos: dc.rights | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | - |
Palavras-chave: dc.subject | Vírus de plantas | - |
Palavras-chave: dc.subject | Metagenômica | - |
Palavras-chave: dc.subject | Picornavirales | - |
Título: dc.title | Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2) : new tentative species within the order picornavirales | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional – UNB |
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