RT-rtPCR quantification of circulating microRNAs in plasma and serum samples from healthy domestic cats

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorScalon, Marcela C.-
Autor(es): dc.creatorMartins, Christine S.-
Autor(es): dc.creatorFerreira, Gabriel G.-
Autor(es): dc.creatorSchlemmer, Franciele-
Autor(es): dc.creatorAlmeida, Ricardo Titze de-
Autor(es): dc.creatorPaludo, Giane Regina-
Data de aceite: dc.date.accessioned2024-10-23T15:33:41Z-
Data de disponibilização: dc.date.available2024-10-23T15:33:41Z-
Data de envio: dc.date.issued2022-05-02-
Data de envio: dc.date.issued2022-05-02-
Data de envio: dc.date.issued2021-07-23-
Fonte completa do material: dc.identifierhttps://repositorio.unb.br/handle/10482/43597-
Fonte completa do material: dc.identifierhttps://doi.org/10.1177/10406387211034843-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-3686-8413-
Fonte completa do material: dc.identifierhttps://orcid.org/ 0000-0002-9112-1718-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/886161-
Descrição: dc.descriptionMicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at a post-transcriptional level by silencing targeted messenger RNA (mRNA). Most studies concerning miRNA expression use solid tissue samples. However, circulating miRNAs from different body fluids have recently emerged as diagnostic and prognostic molecules, given that they hold informative value and have increased stability in cell-free form. Blood sampling of cats can be challenging given their small body size and because they often experience distress when handled. We quantified miR-20a, -192, -365, -15b-5p, and -16-5p from plasma and serum samples of 10 healthy domestic cats. Our RT-rtPCR procedure used 100 µL of either plasma or serum samples as sources of biomarker molecules. However, serum provided higher amounts of miRNA than plasma samples, with a p < 0.0001 for miR-20a and p < 0.0002 for miR-16-5p.-
Descrição: dc.descriptionFaculdade de Agronomia e Medicina Veterinária (FAV)-
Publicador: dc.publisherSAGE-
Relação: dc.relationhttps://journals.sagepub.com/doi/10.1177/10406387211034843-
Direitos: dc.rightsAcesso Restrito-
Palavras-chave: dc.subjectSangue-
Palavras-chave: dc.subjectDiagnóstico-
Palavras-chave: dc.subjectGato - doenças-
Palavras-chave: dc.subjectMicroRNA-
Título: dc.titleRT-rtPCR quantification of circulating microRNAs in plasma and serum samples from healthy domestic cats-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional – UNB

Não existem arquivos associados a este item.