Mesenchymal stem cell-like properties of CD133+ glioblastoma initiating cells

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Autor(es): dc.creatorPavon, Lorena Favaro-
Autor(es): dc.creatorSibov, Tatiana Tais-
Autor(es): dc.creatorOliveira, Daniela Mara de-
Autor(es): dc.creatorMarti, Luciana C.-
Autor(es): dc.creatorCabral, Francisco Romero-
Autor(es): dc.creatorSouza, Jean Gabriel de-
Autor(es): dc.creatorBoufleur, Pamela-
Autor(es): dc.creatorMalheiros, Suzana M. F.-
Autor(es): dc.creatorNeto, Manuel A. de Paiva-
Autor(es): dc.creatorCruz, Edgard Ferreira da-
Autor(es): dc.creatorTavassi, Ana Marisa Chudzinski-
Autor(es): dc.creatorCavalheiro, Sérgio-
Data de aceite: dc.date.accessioned2024-10-23T15:24:22Z-
Data de disponibilização: dc.date.available2024-10-23T15:24:22Z-
Data de envio: dc.date.issued2018-12-11-
Data de envio: dc.date.issued2018-12-11-
Data de envio: dc.date.issued2016-05-
Fonte completa do material: dc.identifierhttp://repositorio.unb.br/handle/10482/33159-
Fonte completa do material: dc.identifierhttps://doi.org/10.18632/oncotarget.9658-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/882245-
Descrição: dc.descriptionGlioblastoma is composed of dividing tumor cells, stromal cells and tumor initiating CD133+ cells. Recent reports have discussed the origin of the glioblastoma CD133+ cells and their function in the tumor microenvironment. The present work sought to investigate the multipotent and mesenchymal properties of primary highly purified human CD133+ glioblastoma-initiating cells. To accomplish this aim, we used the following approaches: i) generation of tumor subspheres of CD133+ selected cells from primary cell cultures of glioblastoma; ii) analysis of the expression of pluripotency stem cell markers and mesenchymal stem cell (MSC) markers in the CD133+ glioblastoma-initiating cells; iii) side-by-side ultrastructural characterization of the CD133+ glioblastoma cells, MSC and CD133+ hematopoietic stem cells isolated from human umbilical cord blood (UCB); iv) assessment of adipogenic differentiation of CD133+ glioblastoma cells to test their MSC-like in vitro differentiation ability; and v) use of an orthotopic glioblastoma xenograft model in the absence of immune suppression. We found that the CD133+ glioblastoma cells expressed both the pluripotency stem cell markers (Nanog, Mush-1 and SSEA-3) and MSC markers. In addition, the CD133+ cells were able to differentiate into adipocyte-like cells. Transmission electron microscopy (TEM) demonstrated that the CD133+ glioblastoma-initiating cells had ultrastructural features similar to those of undifferentiated MSCs. In addition, when administered in vivo to non-immunocompromised animals, the CD133+ cells were also able to mimic the phenotype of the original patient’s tumor. In summary, we showed that the CD133+ glioblastoma cells express molecular signatures of MSCs, neural stem cells and pluripotent stem cells, thus possibly enabling differentiation into both neural and mesodermal cell types.-
Formato: dc.formatapplication/pdf-
Publicador: dc.publisherImpact Journals-
Direitos: dc.rightsAcesso Aberto-
Direitos: dc.rightsOncotarget - All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License (CC BY). Fonte: http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=9658&path[]=41659. Acesso em: 11 dez. 2018.-
Palavras-chave: dc.subjectNeurosferas-
Palavras-chave: dc.subjectGlicoproteínas-
Palavras-chave: dc.subjectCélulas-tronco-
Palavras-chave: dc.subjectMicroscopia eletrônica-
Palavras-chave: dc.subjectTumores-
Título: dc.titleMesenchymal stem cell-like properties of CD133+ glioblastoma initiating cells-
Tipo de arquivo: dc.typelivro digital-
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