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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.creator | Pinto, Larissa G. | - |
Autor(es): dc.creator | Talbot, Jhimmy | - |
Autor(es): dc.creator | Peres, Raphael S. | - |
Autor(es): dc.creator | Franca, Rafael F. | - |
Autor(es): dc.creator | Ferreira, Sérgio Henrique | - |
Autor(es): dc.creator | Ryffel, Bernhard | - |
Autor(es): dc.creator | Alves-Filho, José Carlos F. | - |
Autor(es): dc.creator | Cavalcanti Neto, Florêncio Figueiredo | - |
Autor(es): dc.creator | Cunha, Thiago Mattar | - |
Autor(es): dc.creator | Cunha, Fernando de Queiroz | - |
Data de aceite: dc.date.accessioned | 2024-10-23T15:05:37Z | - |
Data de disponibilização: dc.date.available | 2024-10-23T15:05:37Z | - |
Data de envio: dc.date.issued | 2021-08-14 | - |
Data de envio: dc.date.issued | 2021-08-14 | - |
Data de envio: dc.date.issued | 2015 | - |
Fonte completa do material: dc.identifier | https://repositorio.unb.br/handle/10482/41716 | - |
Fonte completa do material: dc.identifier | https://doi.org/10.1186/s13075-015-0759-2 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/capes/874097 | - |
Descrição: dc.description | Introduction: Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by neutrophil articular infiltration, joint pain and the progressive destruction of cartilage and bone. IL-22 is a key effector molecule that plays a critical role in autoimmune diseases. However, the function of IL-22 in the pathogenesis of RA remains controversial. In this study, we investigated the role of IL-22 in the early phase of antigen-induced arthritis (AIA) in mice. Methods: AIA was induced in C57BL/6, IL-22−/− , ASC−/− and IL-1R1−/− immunized mice challenged intra-articularly with methylated bovine serum albumin (mBSA). Expression of IL-22 in synovial membranes was determined by RT-PCR. Articular hypernociception was evaluated using an electronic von Frey. Neutrophil recruitment and histopathological analyses were assessed in inflamed knee joint. Joint levels of inflammatory mediators and mBSA-specific IgG concentration in the serum were measured by ELISA. Results: The IL-22 mRNA expression and protein levels in synovial tissue were increased during the onset of AIA. In addition, pharmacological inhibition (anti-IL-22 antibody) and genetic deficiency (IL-22−/− mice) reduced articular pain and neutrophil migration in arthritic mice. Consistent with these findings, recombinant IL-22 joint administration promoted articular inflammation per se in WT mice, restoring joint nociception and neutrophil infiltration in IL-22−/− mice. Moreover, IL-22-deficient mice showed reduced synovitis (inflammatory cell influx) and lower joint IL-1β levels, whereas the production of IL-17, MCP-1/CCL2, and KC/CXCL1 and the humoral immune response were similar, compared with WT mice. Corroborating these results, the exogenous administration of IL-22 into the joints induced IL-1β production in WT mice and reestablished IL-1β production in IL-22−/− mice challenged with mBSA. Additionally, IL-1R1−/− mice showed attenuated inflammatory features induced by mBSA or IL-22 challenge. Articular nociception and neutrophil migration induced by IL-22 were also reduced in ASC−/− mice. Conclusions: These results suggest that IL-22 plays a pro-inflammatory/pathogenic role in the onset of AIA through an ASC-dependent stimulation of IL-1β production. | - |
Descrição: dc.description | Faculdade de Medicina (FMD) | - |
Formato: dc.format | application/pdf | - |
Publicador: dc.publisher | BioMed Central | - |
Direitos: dc.rights | Acesso Aberto | - |
Direitos: dc.rights | (CC BY) - © 2015 Pinto et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | - |
Palavras-chave: dc.subject | Ratos | - |
Palavras-chave: dc.subject | Imunização | - |
Palavras-chave: dc.subject | Neutrófilos | - |
Palavras-chave: dc.subject | Artrite reumatoide | - |
Título: dc.title | Joint production of IL-22 participates in the initial phase of antigen-induced arthritis through IL-1β production | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional – UNB |
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