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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.creator | Thiebaut, Flávia | - |
Autor(es): dc.creator | Rojas, Cristian A. | - |
Autor(es): dc.creator | Grativol, Clícia | - |
Autor(es): dc.creator | Calixto, Edmundo P. da R. | - |
Autor(es): dc.creator | Motta, Mariana R. | - |
Autor(es): dc.creator | Ballesteros, Helkin G. F. | - |
Autor(es): dc.creator | Peixoto, Barbara | - |
Autor(es): dc.creator | Lima, Berenice N. S. de | - |
Autor(es): dc.creator | Vieira, Lucas M. | - |
Autor(es): dc.creator | Walter, Maria Emília Machado Telles | - |
Autor(es): dc.creator | Armas, Elvismary M. de | - |
Autor(es): dc.creator | Entenza, Júlio O. P. | - |
Autor(es): dc.creator | Lifschitz, Sergio | - |
Autor(es): dc.creator | Farinelli, Laurent | - |
Autor(es): dc.creator | Hemerly, Adriana Silva | - |
Autor(es): dc.creator | Ferreira, Paulo Cavalcanti Gomes | - |
Data de aceite: dc.date.accessioned | 2024-07-22T13:04:18Z | - |
Data de disponibilização: dc.date.available | 2024-07-22T13:04:18Z | - |
Data de envio: dc.date.issued | 2018-06-26 | - |
Data de envio: dc.date.issued | 2018-06-26 | - |
Data de envio: dc.date.issued | 2017-12-20 | - |
Fonte completa do material: dc.identifier | http://repositorio.unb.br/handle/10482/32109 | - |
Fonte completa do material: dc.identifier | https://doi.org/10.3390/ncrna3040025 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/capes/817179 | - |
Descrição: dc.description | Studies have highlighted the importance of non-coding RNA regulation in plant-microbe interaction. However, the roles of sugarcane microRNAs (miRNAs) in the regulation of disease responses have not been investigated. Firstly, we screened the sRNA transcriptome of sugarcane infected with Acidovorax avenae. Conserved and novel miRNAs were identified. Additionally, small interfering RNAs (siRNAs) were aligned to differentially expressed sequences from the sugarcane transcriptome. Interestingly, many siRNAs aligned to a transcript encoding a coppertransporter gene whose expression was induced in the presence of A. avenae, while the siRNAs were repressed in the presence of A. avenae. Moreover, a long intergenic non-coding RNA was identified as a potential target or decoy of miR408. To extend the bioinformatics analysis, we carried out independent inoculations and the expression patterns of six miRNAs were validated by quantitative reverse transcription-PCR (qRT-PCR). Among these miRNAs, miR408—a copper- microRNA—was downregulated. The cleavage of a putative miR408 target, a laccase, was confirmed by a modified 50RACE (rapid amplification of cDNA ends) assay. MiR408 was also downregulated in samples infected with other pathogens, but it was upregulated in the presence of a beneficial diazotrophic bacteria. Our results suggest that regulation by miR408 is important in sugarcane sensing whether microorganisms are either pathogenic or beneficial, triggering specific miRNA-mediated regulatory mechanisms accordingly. | - |
Formato: dc.format | application/pdf | - |
Publicador: dc.publisher | MDPI | - |
Direitos: dc.rights | Acesso Aberto | - |
Direitos: dc.rights | © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | - |
Palavras-chave: dc.subject | Ácido ribonucléico | - |
Palavras-chave: dc.subject | Bactérias | - |
Palavras-chave: dc.subject | Cana-de-açúcar | - |
Palavras-chave: dc.subject | Biologia computacional | - |
Título: dc.title | Roles of non-coding RNA in sugarcane-microbe interaction | - |
Aparece nas coleções: | Repositório Institucional – UNB |
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