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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.creator | Galetovic, Alexandra | - |
Autor(es): dc.creator | Souza, Renata Torres de | - |
Autor(es): dc.creator | Valiati, Márcia Regina Machado Santos | - |
Autor(es): dc.creator | Cordero Veas, Esteban Mauricio | - |
Autor(es): dc.creator | Bastos, Izabela Marques Dourado | - |
Autor(es): dc.creator | Santana, Jaime Martins de | - |
Autor(es): dc.creator | Ruiz, Jerônimo Conceição | - |
Autor(es): dc.creator | Lima, Fábio Mitsuo | - |
Autor(es): dc.creator | Marini, Marjorie Mendes | - |
Autor(es): dc.creator | Mortara, Renato Arruda | - |
Autor(es): dc.creator | Silveira Filho, José Franco da | - |
Data de aceite: dc.date.accessioned | 2024-07-22T12:43:39Z | - |
Data de disponibilização: dc.date.available | 2024-07-22T12:43:39Z | - |
Data de envio: dc.date.issued | 2013-01-30 | - |
Data de envio: dc.date.issued | 2013-01-30 | - |
Data de envio: dc.date.issued | 2013-01-30 | - |
Data de envio: dc.date.issued | 2010 | - |
Fonte completa do material: dc.identifier | http://repositorio.unb.br/handle/10482/11991 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/capes/810030 | - |
Descrição: dc.description | Paracoccidioides brasiliensis causes paracoccidioidomycosis, a systemic mycosis in Latin America. Formamidases hydrolyze formamide, putatively plays a role infungal nitrogen metabolism. An abundant 45-kDa protein was identified as the P. brasiliensis formamidase. In this study, recombinant formamidase was over-expressed in bacteria and a polyclonal antibody to this protein was produced. Weidentified a 180-kDa protein species reactive to the antibody produced in miceagainst the P. brasiliensis recombinant purified formamidase of 45 kDa. The180-kDa purified protein yielded a heat-denatured species of 45 kDa. Both protein species of 180 and 45 kDa were identified as formamidase by peptide massfinger printing using MS. The identical mass spectra generated by the 180 and the45-kDa protein species indicated that the fungal formamidase is most likely homotetrameric in its native conformation. Furthermore, the purified formami-dase migrated as a protein of 191 kDa in native polyacrylamide gel electrophoresis, thus revealing that the enzyme forms a homotetrameric structure in its native state. This enzyme is present in the fungus cytoplasm and the cell wall. Use of a yeast two-hybrid system revealed cell wall membrane proteins, in addition to cytosolic proteins interacting with formamidase. These data provide new insights intoformamidase structure as well as potential roles for formamidase and its interaction partners in nitrogen metabolism. | - |
Formato: dc.format | application/pdf | - |
Direitos: dc.rights | Acesso Aberto | - |
Direitos: dc.rights | © 2011 Galetovic et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | - |
Palavras-chave: dc.subject | Tripanossoma cruzi | - |
Palavras-chave: dc.subject | Genética | - |
Palavras-chave: dc.subject | Chagas, Doença de | - |
Título: dc.title | The repetitive cytoskeletal protein H49 of Trypanosoma cruzi is a calpain-like protein located at the flagellum attachment zone | - |
Aparece nas coleções: | Repositório Institucional – UNB |
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