Use of an on/of tetracycline riboswitch to control protein production in Komagataella phafi

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Autor(es): dc.contributorUniversidade de Brasília, Instituto de Ciências Biológicas, Laboratório de Biologia Molecular-
Autor(es): dc.contributorUniversidade Federal do Rio de Janeiro, Instituto de Bioquímica Médica, Laboratório de Biotecnologia de Leveduras-
Autor(es): dc.contributorUniversidade de Brasília, Instituto de Ciências Biológicas, Laboratório de Biologia Sintética-
Autor(es): dc.contributorUniversidade de Brasília, Instituto de Ciências Biológicas, Laboratório de Biologia Molecular-
Autor(es): dc.contributorUniversidade de Brasília, Instituto de Ciências Biológicas, Laboratório de Biologia Molecular-
Autor(es): dc.contributorUniversidade de Brasília, Instituto de Ciências Biológicas, Laboratório de Biologia Molecular-
Autor(es): dc.creatorVieira, Igor Patrick Vasconcelos-
Autor(es): dc.creatorPimentel, Felipe Seixas Arreguy-
Autor(es): dc.creatorCoelho, Cintia Marques-
Autor(es): dc.creatorDe Marco, Janice Lisboa-
Autor(es): dc.creatorMoraes, Lidia Maria Pepe de-
Autor(es): dc.creatorTorres, Fernando Araripe Gonçalves-
Data de aceite: dc.date.accessioned2024-07-22T12:21:48Z-
Data de disponibilização: dc.date.available2024-07-22T12:21:48Z-
Data de envio: dc.date.issued2024-02-06-
Data de envio: dc.date.issued2024-02-06-
Data de envio: dc.date.issued2023-11-20-
Fonte completa do material: dc.identifierhttp://repositorio2.unb.br/jspui/handle/10482/47704-
Fonte completa do material: dc.identifierhttps://doi.org/10.1186/s13568-023-01637-5-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-3650-7271-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/802031-
Descrição: dc.descriptionThe methylotrophic yeast Komagataella phafi is one of the most important microbial platforms to produce recom binant proteins. Despite its importance in the context of industrial biotechnology, the use of synthetic biology approaches in K. phafi is hampered by the fact that few genetic tools are available for precise control of gene expres sion in this system. In this work, we used an RNA aptamer activated by tetracycline to modulate protein production at the translational level. Using lacZ as gene reporter, we have demonstrated signifcant reduction of the heterolo gous protein upon addition of tetracycline. Furthermore, this genetic control device was applied for the control of Ku70p. This protein is involved in non-homologous recombination and the control of its production paves the way for the development of strains exhibiting higher rates of homologous recombination.-
Descrição: dc.descriptionInstituto de Ciências Biológicas (IB)-
Descrição: dc.descriptionDepartamento de Biologia Celular (IB CEL)-
Descrição: dc.descriptionDepartamento de Genética e Morfologia (IB GEM)-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Publicador: dc.publisherAMB Express-
Direitos: dc.rightsAcesso Aberto-
Direitos: dc.rights© The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.-
Palavras-chave: dc.subjectLeveduras-
Palavras-chave: dc.subjectTetraciclina-
Palavras-chave: dc.subjectProteínas-
Título: dc.titleUse of an on/of tetracycline riboswitch to control protein production in Komagataella phafi-
Aparece nas coleções:Repositório Institucional – UNB

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