Effects of in vitro short- and long-term treatment with telomerase inhibitor in U-251 glioma cells

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Autor(es): dc.contributorUniversidade de Brasília, Faculdade UnB Ceilândia-
Autor(es): dc.contributorUniversidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia-
Autor(es): dc.contributorUniversidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia-
Autor(es): dc.contributorUniversidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia-
Autor(es): dc.contributorLaboratório de Hemato-oncologia Celular e Molecular, Instituto Nacional do Câncer (INCA)-
Autor(es): dc.contributorDepartamento de Biotecnologia Marinha, Instituto de Estudos do Mar do Almirante Paulo Moreira, IEAPM-
Autor(es): dc.creatorMota, Tales Henrique Andrade da-
Autor(es): dc.creatorGuimarães, Ana Flavia Reis-
Autor(es): dc.creatorCarvalho, Amandda Évelin Silva de-
Autor(es): dc.creatorAraújo, Felipe Saldanha de-
Autor(es): dc.creatorLopes, Giselle Pinto de Faria-
Autor(es): dc.creatorPittella-Silva, Fabio-
Autor(es): dc.creatorRabello, Doralina do Amaral-
Autor(es): dc.creatorOliveira, Diego Madureira de-
Data de aceite: dc.date.accessioned2024-07-22T12:11:44Z-
Data de disponibilização: dc.date.available2024-07-22T12:11:44Z-
Data de envio: dc.date.issued2022-09-05-
Data de envio: dc.date.issued2022-09-05-
Data de envio: dc.date.issued2020-
Fonte completa do material: dc.identifierhttps://repositorio.unb.br/handle/10482/44690-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-6272-6558-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/798261-
Descrição: dc.descriptionBACKGROUND: The inhibition of the enzyme telomerase (TERT) has been widely investigated as a new pharmacological approach for cancer treatment, but its real potential and the biochemical consequences are not totally understood. OBJECTIVE: Here, we investigated the effects of the telomerase inhibitor MST-312 on a human glioma cell line after both short- and long-term (290 days) treatments. METHODS: Effects on cell growth, viability, cell cycle, morphology, cell death and genes expression were assessed. RESULTS: We found that short-term treatment promoted cell cycle arrest followed by apoptosis. Importantly, cells with telomerase knock-down revealed that the toxic effects of MST-312 are partially TERT dependent. In contrast, although the long-term treatment decreased cell proliferation at first, it also caused adaptations potentially related to treatment resistance and tumor aggressiveness after long time of exposition. CONCLUSIONS: Despite the short-term effects of telomerase inhibition not being due to telomere erosion, they are at least partially related to the enzyme inhibition, which may represent an important strategy to pave the way for tumor growth control, especially through modulation of the non-canonical functions of telomerase. On the other hand, long-term exposure to the inhibitor had the potential to induce cell adaptations with possible negative clinical implications.-
Formato: dc.formatapplication/pdf-
Publicador: dc.publisherIOS Press-
Direitos: dc.rightsAcesso Aberto-
Direitos: dc.rights© 2021 – The authors. Published by IOS Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC 4.0)-
Palavras-chave: dc.subjectGlioma-
Palavras-chave: dc.subjectTelomerase-
Palavras-chave: dc.subjectCâncer - tratamento-
Título: dc.titleEffects of in vitro short- and long-term treatment with telomerase inhibitor in U-251 glioma cells-
Aparece nas coleções:Repositório Institucional – UNB

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