L-Asparaginase from Penicillium sizovae produced by a recombinant Komagataella phaffii strain

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Autor(es): dc.contributorUniversity of Brasilia, Health Sciences School-
Autor(es): dc.contributorUniversity of Brasilia, Health Sciences School-
Autor(es): dc.contributorUniversity of Brasilia, Health Sciences School-
Autor(es): dc.contributorUniversity of Brasilia, Health Sciences School-
Autor(es): dc.contributorUniversity of Brasilia, Health Sciences School-
Autor(es): dc.contributorUniversity of Brasilia, Institute of Biological Sciences-
Autor(es): dc.contributorUniversity of Sao Paulo, Department of Biochemical and Pharmaceutical Technology-
Autor(es): dc.contributorUniversity of Brighton, School of Pharmacy and Biomolecular Sciences-
Autor(es): dc.contributorUniversity of Brighton, School of Pharmacy and Biomolecular Sciences-
Autor(es): dc.contributorUniversity of Brighton, School of Pharmacy and Biomolecular Sciences-
Autor(es): dc.contributorUniversity of Brasilia, Health Sciences School-
Autor(es): dc.creatorFreitas, Marcela Medeiros de-
Autor(es): dc.creatorSouza, Paula Monteiro de-
Autor(es): dc.creatorHomem de Mello, Mauricio-
Autor(es): dc.creatorFonseca-Bazzo, Yris Maria-
Autor(es): dc.creatorSilveira, Dâmaris-
Autor(es): dc.creatorFerreira Filho, Edivaldo Ximenes-
Autor(es): dc.creatorPessoa Junior, Adalberto-
Autor(es): dc.creatorSarker, Dipak-
Autor(es): dc.creatorTimson, David-
Autor(es): dc.creatorInácio, João-
Autor(es): dc.creatorMagalhães, Pérola de Oliveira-
Data de aceite: dc.date.accessioned2024-07-22T12:07:40Z-
Data de disponibilização: dc.date.available2024-07-22T12:07:40Z-
Data de envio: dc.date.issued2024-07-16-
Data de envio: dc.date.issued2024-07-16-
Data de envio: dc.date.issued2022-06-14-
Fonte completa do material: dc.identifierhttp://repositorio2.unb.br/jspui/handle/10482/48896-
Fonte completa do material: dc.identifierhttps://doi.org/10.3390/ph15060746-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-6112-9903-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0003-1824-2211-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-4541-9177-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-1230-3207-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0003-1851-5224-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0003-4947-8753-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-5268-8690-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-2912-415X-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-0985-8818-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0001-8011-6940-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/796588-
Descrição: dc.description: L-asparaginase is an important enzyme in the pharmaceutical field used as treatment for acute lymphoblastic leukemia due to its ability to hydrolyze L-asparagine, an essential amino acid synthesized by normal cells, but not by neoplastic cells. Adverse effects of L-asparaginase formulations are associated with its glutaminase activity and bacterial origin; therefore, it is important to find new sources of L-asparaginase produced by eukaryotic microorganisms with low glutaminase activity. This work aimed to identify the L-asparaginase gene sequence from Penicillium sizovae, a filamentous fungus isolated from the Brazilian Savanna (Cerrado) soil with low glutaminase activity, and to biosynthesize higher yields of this enzyme in the yeast Komagataella phaffii. The L-asparaginase gene sequence of P. sizovae was identified by homology to L-asparaginases from species of Penicillium of the section Citrina: P. citrinum and P. steckii. Partial L-asparaginase from P. sizovae, lacking the periplasmic signaling sequence, was cloned, and expressed intracellularly with highest enzymatic activity achieved by a MUT+ clone cultured in BMM expression medium; a value 5-fold greater than that obtained by native L-asparaginase in P. sizovae cells. To the best of our knowledge, this is the first literature report of the heterologous production of an L-asparaginase from a filamentous fungus by a yeast.-
Descrição: dc.descriptionFaculdade de Ciências da Saúde (FS)-
Descrição: dc.descriptionDepartamento de Farmácia (FS FAR)-
Descrição: dc.descriptionInstituto de Ciências Biológicas (IB)-
Descrição: dc.descriptionDepartamento de Biologia Celular (IB CEL)-
Descrição: dc.descriptionPrograma de Pós-Graduação em Ciências Farmacêuticas-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Publicador: dc.publisherMDPI-
Direitos: dc.rightsAcesso Aberto-
Palavras-chave: dc.subjectL-Asparaginase-
Palavras-chave: dc.subjectLeucemia linfoblástica aguda (LLA)-
Palavras-chave: dc.subjectClonagem-
Palavras-chave: dc.subjectFungos-
Título: dc.titleL-Asparaginase from Penicillium sizovae produced by a recombinant Komagataella phaffii strain-
Aparece nas coleções:Repositório Institucional – UNB

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