The peptide secreted at the water to land transition in a model amphibian has antioxidant effects

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorBarbosa, Eder Alves-
Autor(es): dc.creatorPlácido, Alexandra-
Autor(es): dc.creatorMoreira, Daniel Carneiro-
Autor(es): dc.creatorAlbuquerque, Lucas Fraga Friaça-
Autor(es): dc.creatorDematei, Anderson-
Autor(es): dc.creatorCarvalho, Amandda Évelin Silva de-
Autor(es): dc.creatorCabral, Wanessa F.-
Autor(es): dc.creatorBáo, Sônia Nair-
Autor(es): dc.creatorAraújo, Felipe Saldanha de-
Autor(es): dc.creatorKuckelhaus, Selma Aparecida Souza-
Autor(es): dc.creatorBorges, Tatiana K.-
Autor(es): dc.creatorPortugal, Camila C.-
Autor(es): dc.creatorSocodato, Renato-
Autor(es): dc.creatorTeixeira, Cátia-
Autor(es): dc.creatorLima, Filipe Camargo D. A.-
Autor(es): dc.creatorBatagin-Neto, Augusto-
Autor(es): dc.creatorSebben, Antônio-
Autor(es): dc.creatorEaton, Peter-
Autor(es): dc.creatorGomes, Paula-
Autor(es): dc.creatorBrand, Guilherme D.-
Autor(es): dc.creatorRelvas, João B.-
Autor(es): dc.creatorKato, Massuo J.-
Autor(es): dc.creatorLeite, José Roberto de Souza de Almeida-
Data de aceite: dc.date.accessioned2024-07-22T12:05:03Z-
Data de disponibilização: dc.date.available2024-07-22T12:05:03Z-
Data de envio: dc.date.issued2022-02-06-
Data de envio: dc.date.issued2022-02-06-
Data de envio: dc.date.issued2021-11-09-
Fonte completa do material: dc.identifierhttps://repositorio.unb.br/handle/10482/42820-
Fonte completa do material: dc.identifierhttps://doi.org/10.1098/rspb.2021.1531-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/795618-
Descrição: dc.descriptionIn addition to the morphophysiological changes experienced by amphibians during metamorphosis, they must also deal with a different set of environmental constraints when they shift from the water to the land. We found that Pithecopus azureus secretes a single peptide ([M + H]+ = 658.38 Da) at the developmental stage that precedes the onset of terrestrial behaviour. De novo peptide and cDNA sequencing revealed that the peptide, named PaT-2, is expressed in tandem and is a member of the tryptophyllins family. In silico studies allowed us to identify the position of reactive sites and infer possible antioxidant mechanisms of the compounds. Cell-based assays confirmed the predicted antioxidant activity in mammalian microglia and neuroblast cells. The potential neuroprotective effect of PaT-2 was further corroborated in FRET-based live cell imaging assays, where the peptide prevented lipopolysaccharide-induced ROS production and glutamate release in human microglia. In summary, PaT-2 is the first peptide expressed during the ontogeny of P. azureus, right before the metamorphosing froglet leaves the aquatic environment to occupy terrestrial habitats. The antioxidant activity of PaT-2, predicted by in silico analyses and confirmed by cell-based assays, might be relevant for the protection of the skin of P. azureus adults against increased O2 levels and UV exposure on land compared with aquatic environments.-
Descrição: dc.descriptionFaculdade de Medicina (FMD)-
Publicador: dc.publisherThe Royal Society-
Relação: dc.relationhttps://royalsocietypublishing.org/doi/10.1098/rspb.2021.1531-
Direitos: dc.rightsAcesso Aberto-
Palavras-chave: dc.subjectAnfíbio-
Palavras-chave: dc.subjectTriptofilinas-
Palavras-chave: dc.subjectPeptídeos-
Palavras-chave: dc.subjectEspectrometria de massa-
Palavras-chave: dc.subjectStress oxidativo-
Título: dc.titleThe peptide secreted at the water to land transition in a model amphibian has antioxidant effects-
Aparece nas coleções:Repositório Institucional – UNB

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