Plasma or serum : which Is preferable for mutation detection in liquid biopsy?

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de Brasília, Faculdade de Ciências da Saúde-
Autor(es): dc.contributorCancer Precision Medicine Center, Japanese Foundation for Cancer Research, Tokyo, Japan-
Autor(es): dc.contributorCancer Precision Medicine Center, Japanese Foundation for Cancer Research, Tokyo, Japan-
Autor(es): dc.contributorDepartment of Gastroenterological Surgery, Cancer Institute Hospital, Japanese Foundation for Cancer Research, Tokyo, Japan-
Autor(es): dc.contributorDepartment of Respiratory Medicine, Tohoku University, Sendai, Japan-
Autor(es): dc.contributorCancer Precision Medicine Center, Japanese Foundation for Cancer Research, Tokyo, Japan-
Autor(es): dc.contributorCancer Precision Medicine Center, Japanese Foundation for Cancer Research, Tokyo, Japan-
Autor(es): dc.creatorPittella-Silva, Fabio-
Autor(es): dc.creatorYoon Ming Chin-
Autor(es): dc.creatorHiu Ting Chan-
Autor(es): dc.creatorNagayama, Satoshi-
Autor(es): dc.creatorMiyauchi, Eisaku-
Autor(es): dc.creatorLow, Siew-Kee-
Autor(es): dc.creatorNakamura, Yusuke-
Data de aceite: dc.date.accessioned2024-07-22T11:39:24Z-
Data de disponibilização: dc.date.available2024-07-22T11:39:24Z-
Data de envio: dc.date.issued2022-09-29-
Data de envio: dc.date.issued2022-09-29-
Data de envio: dc.date.issued2020-06-09-
Fonte completa do material: dc.identifierhttps://repositorio.unb.br/handle/10482/44933-
Fonte completa do material: dc.identifierhttps://doi.org/10.1093/clinchem/hvaa103-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/785727-
Descrição: dc.descriptionBackground Blood-based analysis of circulating tumor DNA (ctDNA) is a promising tool for cancer screening, monitoring relapse/recurrence and evaluating response to treatment. Although plasma is widely used to obtain ctDNA, biorepositories worldwide possess a huge number of serum samples and comparative studies on the use of serum vs plasma as ctDNA sources are essential. Methods We analyzed cell-free DNA (cfDNA) from matched EDTA-plasma and serum samples from healthy donors and patients with colorectal or lung cancer, and used targeted next-generation sequencing to evaluate mutation detection efficiency and reproducibility. Matched samples from healthy individuals were spiked with reference oligonucleotides and sequenced using the Ion-S5 Oncomine-Pan-Cancer panel. Detection efficiency in matched samples from patients with cancer was evaluated using 2 distinct gene panels and compared to mutations found in tissue-biopsy samples at diagnosis. Results Mean total cfDNA was 55% higher in serum samples and the presence of longer DNA fragments was significantly increased in serum compared with plasma samples (P = 0.0001 to 0.015). Spiked mutated nucleotides were detected in both samples, but allele frequencies (AF) were approximately half in serum compared with plasma, suggesting ctDNA from serum was more diluted by DNA of noncancerous origins. Matched samples from patients with cancer revealed that up to 44.8% of mutations with low AF were missed in serum samples and concordance rates with somatic mutations found in tissue biopsy at diagnosis was better in plasma samples. Conclusion The use of serum in retrospective studies should consider the limitations for detecting low AF mutations. Plasma is clearly preferable for prospective clinical applications of liquid biopsy.-
Publicador: dc.publisherOxford University Press-
Relação: dc.relationhttps://academic.oup.com/clinchem/article/66/7/946/5855218-
Direitos: dc.rightsAcesso Restrito-
Palavras-chave: dc.subjectPlasma-
Palavras-chave: dc.subjectSoro-
Palavras-chave: dc.subjectMutação genética-
Palavras-chave: dc.subjectBiópsia líquida-
Título: dc.titlePlasma or serum : which Is preferable for mutation detection in liquid biopsy?-
Aparece nas coleções:Repositório Institucional – UNB

Não existem arquivos associados a este item.