Amplification of mutant KRASG12D in a patient with advanced metastatic pancreatic adenocarcinoma detected by liquid biopsy : a case report

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Autor(es): dc.creatorPittella-Silva, Fabio-
Autor(es): dc.creatorKimura, Yasutoshi-
Autor(es): dc.creatorLow, Siew‑Kee-
Autor(es): dc.creatorNakamura, Yusuke-
Autor(es): dc.creatorMotoya, Masayo-
Data de aceite: dc.date.accessioned2024-07-22T11:33:56Z-
Data de disponibilização: dc.date.available2024-07-22T11:33:56Z-
Data de envio: dc.date.issued2022-09-05-
Data de envio: dc.date.issued2022-09-05-
Data de envio: dc.date.issued2020-
Fonte completa do material: dc.identifierhttps://repositorio.unb.br/handle/10482/44689-
Fonte completa do material: dc.identifierhttps://doi.org/10.3892/mco.2021.2334-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/783519-
Descrição: dc.descriptionPancreatic ductal adenocarcinoma (PDAC) remains one of the deadliest cancer types. Activating oncogenic KRAS mutations are commonly observed in PDAC; however, oncogenic KRAS amplification is rarely observed, and its significance in prognosis and resistance to therapy remains poorly characterized. The present report describes the case of a 52‑year‑old male patient diagnosed with advanced PDAC with liver metastasis. The patient received modified FOLFIRINOX (mFFX) therapy to which the patient became intolerant with a strong inflammatory response. Subsequent treatment with gemcitabine plus nab‑paclitaxel failed to control the disease. Targeted genetic analysis revealed KRASG12D and TP53R248Q mutations in the primary tumor and liver metastases. Analysis of circulating tumor DNA (ctDNA) before the first line of treat‑ ment confirmed these genetic findings and revealed a >4‑fold amplification of the mutant KRASG12D not detected in the primary tumor. Additionally, subsequent analysis confirmed a 5‑fold amplification of the KRASG12D allele in liver metastasis. Consecutive monitoring of ctDNA revealed an initial decrease in the tumor burden 2 weeks after the first cycle of mFFX. However, coinciding with treatment intolerance, a sharp increase in tumor mutational levels and KRASG12D amplifica‑ tion was observed 1 month later. The patient died 70 days after treatment initiation. Overall, amplification of oncogenic KRASG12D was not only associated with an aggressive pheno‑ type, but also supported cancer resistance to chemotherapy. Importantly, this case suggests that plasma detection of KRASG12D amplification is feasible in the clinical routine and constitutes a powerful tool for assessing tumor aggressiveness.-
Formato: dc.formatapplication/pdf-
Publicador: dc.publisherSpandidos Publications-
Direitos: dc.rightsAcesso Aberto-
Direitos: dc.rights(CC BY NC ND) This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License.-
Palavras-chave: dc.subjectBiópsia líquida-
Palavras-chave: dc.subjectDiagnóstico-
Palavras-chave: dc.subjectAdenocarcinoma pancreático ductal-
Palavras-chave: dc.subjectPrognóstico-
Palavras-chave: dc.subjectTumores-
Título: dc.titleAmplification of mutant KRASG12D in a patient with advanced metastatic pancreatic adenocarcinoma detected by liquid biopsy : a case report-
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