α-Alkylidene δ-lactones inhibit quorum sensing phenotypes in Chromobacterium strain CV026 showing interaction with the CviR receptor

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorFavero, Fernanda-
Autor(es): dc.creatorTolentino, Terezinha Alves-
Autor(es): dc.creatorFernandes, Vinicius-
Autor(es): dc.creatorTreptow, Werner-
Autor(es): dc.creatorPereira, Alex Leite-
Autor(es): dc.creatorMachado, Angelo Henrique Lira-
Data de aceite: dc.date.accessioned2024-07-22T11:32:31Z-
Data de disponibilização: dc.date.available2024-07-22T11:32:31Z-
Data de envio: dc.date.issued2023-11-27-
Data de envio: dc.date.issued2023-11-27-
Data de envio: dc.date.issued2023-06-15-
Fonte completa do material: dc.identifierhttp://repositorio2.unb.br/jspui/handle/10482/46916-
Fonte completa do material: dc.identifierhttps://doi.org/10.1039/D3RA01975F-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0003-4284-9929-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/782903-
Descrição: dc.descriptionDisruption of bacterial quorum sensing (QS) is presented as a promising strategy to overcome clinically relevant and phytopathogenic bacteria. This work presents α-alkylidene δ-lactones as new chemical scaffolds that inhibit the biosynthesis of violacein in the biosensor strain Chromobacterium CV026. Three molecules displayed higher than 50% violacein reduction when tested at concentrations lower than 625 µM. The most active α-alkylidene δ-lactone inhibited the hydrolysis of chitin concomitantly with the inhibition of violacein production in CV026, suggesting the disruption of its QS machinery. Further, RT-qPCR and competition experiments showed this molecule to be a transcriptional inhibitor of the QS-regulated operon vioABCDE. Docking calculations suggested a good correlation between binding affinity energies and inhibition effects, with all molecules positioned within the CviR autoinducer-binding domain (AIBD). The most active lactone yielded the best binding affinity energy, most probably due to its unprecedented binding with the AIBD. Our results show α-alkylidene δ-lactones as promising chemical scaffolds for the development of new QS inhibitors affecting LuxR/LuxI-systems.-
Descrição: dc.descriptionInstituto de Química (IQ)-
Descrição: dc.descriptionFaculdade UnB Ceilândia (FCE)-
Descrição: dc.descriptionInstituto de Ciências Biológicas (IB)-
Descrição: dc.descriptionDepartamento de Biologia Celular (IB CEL)-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Publicador: dc.publisherThe Royal Society of Chemistry-
Direitos: dc.rightsAcesso Aberto-
Direitos: dc.rights(CC BY NC) This article is licensed under aCreative Commons Attribution-NonCommercial 3.0 Unported Licence.-
Palavras-chave: dc.subjectBactérias-
Palavras-chave: dc.subjectLactonas-
Título: dc.titleα-Alkylidene δ-lactones inhibit quorum sensing phenotypes in Chromobacterium strain CV026 showing interaction with the CviR receptor-
Aparece nas coleções:Repositório Institucional – UNB

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