NFAT2 isoforms differentially regulate gene expression, cell death, and transformation through alternative n-terminal domains

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorNóbrega, Pedro Ivo Lucena da-
Autor(es): dc.creatorFaget, Douglas V.-
Autor(es): dc.creatorPachulec, Emilia-
Autor(es): dc.creatorRobaina, Marcela C.-
Autor(es): dc.creatorKlumb, Claudete E.-
Autor(es): dc.creatorRobbs, Bruno K.-
Autor(es): dc.creatorViola, João P. B.-
Data de aceite: dc.date.accessioned2024-07-11T17:51:01Z-
Data de disponibilização: dc.date.available2024-07-11T17:51:01Z-
Data de envio: dc.date.issued2023-05-16-
Data de envio: dc.date.issued2023-05-16-
Data de envio: dc.date.issued2016-
Fonte completa do material: dc.identifierhttp://app.uff.br/riuff/handle/1/28833-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/758767-
Descrição: dc.descriptionSim-
Descrição: dc.descriptionThe NFAT (nuclear factor of activated T cells) family of transcription factors is composed of four calcium-responsive proteins (NFAT1 to -4). The NFAT2 (also called NFATc1) gene encodes the isoforms NFAT2 and NFAT2 that result mainly from alternative initiation exons that provide two different N-terminal transactivation domains. However, the specific roles of the NFAT2 isoforms in cell physiology remain unclear. Because previous studies have shown oncogenic potential for NFAT2, this study emphasized the role of the NFAT2 isoforms in cell transformation. Here, we show that a constitutively active form of NFAT2 (CANFAT2 ) and CA-NFAT2 distinctly control death and transformation in NIH 3T3 cells. While CA-NFAT2 strongly induces cell transformation, CA-NFAT2 leads to reduced cell proliferation and intense cell death through the upregulation of tumor necrosis factor alpha (TNF- ). CA-NFAT2 also increases cell death and upregulates Fas ligand (FasL) and TNF- in CD4 T cells. Furthermore, we demonstrate that differential roles of NFAT2 isoforms in NIH 3T3 cells depend on the N-terminal domain, where the NFAT2 -specific N-terminal acidic motif is necessary to induce cell death. Interestingly, the NFAT2 isoform is upregulated in Burkitt lymphomas, suggesting an isoform-specific involvement of NFAT2 in cancer development. Finally, our data suggest that alternative N-terminal domains of NFAT2 could provide differential mechanisms for the control of cellular functions.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Publicador: dc.publisherTaylor and Francis-
Publicador: dc.publisherNiterói, RJ-
Relação: dc.relationMOLECULAR AND CELLULAR BIOLOGY. London: Taylor and Francis, 1981-2023. Mensal. ISSN: 1098-5549-
Direitos: dc.rightsOpen Access-
Direitos: dc.rightsCC-BY-SA-
Palavras-chave: dc.subjectIsoformas de proteínas-
Palavras-chave: dc.subjectFatores de Transcrição NFATC-
Título: dc.titleNFAT2 isoforms differentially regulate gene expression, cell death, and transformation through alternative n-terminal domains-
Título: dc.titleNFAT2 isoforms differentially regulate gene expression, cell death, and transformation through alternative n-terminal domains-
Tipo de arquivo: dc.typeArtigo de periódicos-
Aparece nas coleções:Repositório Institucional da Universidade Federal Fluminense - RiUFF

Não existem arquivos associados a este item.