A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics

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Autor(es): dc.creatorLavich, Tatiana Ramos-
Autor(es): dc.creatorCordeiro, Renato Sergio Balao-
Autor(es): dc.creatorSilva, Patricia Machado Rodrigues e Silva-
Autor(es): dc.creatorMartins, Marco Aurélio-
Data de aceite: dc.date.accessioned2021-10-14T18:32:36Z-
Data de disponibilização: dc.date.available2021-10-14T18:32:36Z-
Data de envio: dc.date.issued2012-12-14-
Data de envio: dc.date.issued2012-12-14-
Data de envio: dc.date.issued2005-03-
Fonte completa do material: dc.identifierhttp://repositorio.unb.br/handle/10482/11813-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2005000300016-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/634168-
Descrição: dc.descriptionIt is widely accepted that the classical constant-temperature hot-plate test is insensitive to cyclooxygenase inhibitors. In the current study, we developed a variant of the hot-plate test procedure (modified hot-plate (MHP) test) to measure inflammatory nociception in freely moving rats and mice. Following left and right hind paw stimulation with a phlogogen and vehicle, respectively, the animals were placed individually on a hot-plate surface at 51ºC and the withdrawal latency for each paw was determined simultaneously in measurements performed at 15, 60, 180, and 360 min post-challenge. Plantar stimulation of rats (250 and 500 µg/paw) and mice (125-500 µg/paw) with carrageen an led to a rapid hyperalgesic response of the ipsilateral paw that reached a plateau from 15 to 360 min after challenge. Pretreatment with indomethacin (4 mg/kg, ip) inhibited the phenomenon at all the times analyzed. Similarly, plantar stimulation of rats and mice with prostaglandin E2 (0.5 and 1 µg/paw) also resulted in rapid hyperalgesia which was first detected 15 min post-challenge. Finally, we observed that the MHP test was more sensitive than the classical Hargreaves' test, being able to detect about 4- and 10-fold lower doses of prostaglandin E2 and carrageenan, respectively. In conclusion, the MHP test is a simple and sensitive method for detecting peripheral hyperalgesia and analgesia in rats and mice. This test represents a low-cost alternative for the study of inflammatory pain in freely moving animals.-
Formato: dc.formatapplication/pdf-
Publicador: dc.publisherAssociação Brasileira de Divulgação Científica-
Direitos: dc.rightsAcesso Aberto-
Direitos: dc.rightsBrazilian Journal of Medical and Biological Research - Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons (Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)). Fonte: http://www.scielo.br/scielo.php?script=sci_serial&pid=0100-879X&lng=pt&nrm=iso. Acesso em: 7 nov. 2012.-
Palavras-chave: dc.subjectDor-
Palavras-chave: dc.subjectMedição da dor-
Palavras-chave: dc.subjectAnalgesia-
Palavras-chave: dc.subjectHiperalgesia-
Título: dc.titleA novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional – UNB

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