In vivo trans-specific gene silencing in fungal cells by in planta expression of a double-stranded RNA

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorTinoco, Maria Laine Penha-
Autor(es): dc.creatorDias, Bárbara B. A.-
Autor(es): dc.creatorDall’Astta, Rebeca C.-
Autor(es): dc.creatorPamphile, João Alencar-
Autor(es): dc.creatorAragão, Francisco José Lima-
Data de aceite: dc.date.accessioned2021-10-14T18:17:51Z-
Data de disponibilização: dc.date.available2021-10-14T18:17:51Z-
Data de envio: dc.date.issued2017-07-17-
Data de envio: dc.date.issued2017-07-17-
Data de envio: dc.date.issued2010-03-31-
Fonte completa do material: dc.identifierhttp://repositorio.unb.br/handle/10482/23842-
Fonte completa do material: dc.identifierhttps://dx.doi.org/10.1186/1741-7007-8-27-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/628211-
Descrição: dc.descriptionBackground: Self-complementary RNA transcripts form a double-stranded RNA (dsRNA) that triggers a sequencespecific mRNA degradation, in a process known as RNA interference (RNAi), leading to gene silencing. In vascular plants, RNAi molecules trafficking occur between cells and systemically throughout the plant. RNAi signals can spread systemically throughout a plant, even across graft junctions from transgenic to non-transgenic stocks. There is also a great interest in applying RNAi to pathogenic fungi. Specific inhibition of gene expression by RNAi has been shown to be suitable for a multitude of phytopathogenic filamentous fungi. However, double-stranded (ds) RNA/small interfering (si)RNA silencing effect has not been observed in vivo. Results: This study demonstrates for the first time the in vivo interference phenomenon in the pathogenic fungus Fusarium verticillioides, in which expression of an individual fungal transgene was specifically abolished by inoculating mycelial cells in transgenic tobacco plants engineered to express siRNAs from a dsRNA corresponding to the particular transgene. Conclusion: The results provide a powerful tool for further studies on molecular plant-microbe and symbiotic interactions. From a biotechnological perspective, silencing of fungal genes by generating siRNAs in the host provides a novel strategy for the development of broad fungi-resistance strategies in plants and other organisms.-
Formato: dc.formatapplication/pdf-
Publicador: dc.publisherBioMed Central-
Direitos: dc.rightsAcesso Aberto-
Direitos: dc.rights© 2010 Tinoco et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.-
Palavras-chave: dc.subjectRNA-
Palavras-chave: dc.subjectSilenciamento gênico-
Palavras-chave: dc.subjectPlantas-
Palavras-chave: dc.subjectFungos patogênicos-
Título: dc.titleIn vivo trans-specific gene silencing in fungal cells by in planta expression of a double-stranded RNA-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional – UNB

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