Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2) : new tentative species within the order picornavirales

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Autor(es): dc.creatorNery, Flávia M. B.-
Autor(es): dc.creatorMelo, Fernando Lucas-
Autor(es): dc.creatorBoiteux, Leonardo Silva-
Autor(es): dc.creatorRibeiro, Simone G.-
Autor(es): dc.creatorResende, Renato de Oliveira-
Autor(es): dc.creatorOrílio, Anelise Franco-
Autor(es): dc.creatorBatista, Josiane Goulart-
Autor(es): dc.creatorLima, Mirtes Freitas-
Autor(es): dc.creatorCarvalho, Rita de Cássia Pereira-
Data de aceite: dc.date.accessioned2021-10-14T18:00:35Z-
Data de disponibilização: dc.date.available2021-10-14T18:00:35Z-
Data de envio: dc.date.issued2020-10-21-
Data de envio: dc.date.issued2020-10-21-
Data de envio: dc.date.issued2020-08-27-
Fonte completa do material: dc.identifierhttps://repositorio.unb.br/handle/10482/39576-
Fonte completa do material: dc.identifierhttps://doi.org/10.3390/v12090950-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-5791-8002-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-4965-1424-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/621430-
Descrição: dc.descriptionIn a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered.-
Formato: dc.formatapplication/pdf-
Publicador: dc.publisherMDPI-
Direitos: dc.rightsAcesso Aberto-
Direitos: dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).-
Palavras-chave: dc.subjectVírus de plantas-
Palavras-chave: dc.subjectMetagenômica-
Palavras-chave: dc.subjectPicornavirales-
Título: dc.titleMolecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2) : new tentative species within the order picornavirales-
Tipo de arquivo: dc.typelivro digital-
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