Cellular cholesterol efflux mediated by HDL isolated from subjects with low HDL levels and Coronary artery disease

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorUint, Luciana-
Autor(es): dc.creatorSpósito, Andrei Carvalho-
Autor(es): dc.creatorBrandizzi, Laura Ines Ventura-
Autor(es): dc.creatorYoshida, Vanda Mitie-
Autor(es): dc.creatorMaranhão, Raul Cavalcante-
Autor(es): dc.creatorLuz, Protásio Lemos da-
Data de aceite: dc.date.accessioned2021-10-14T17:58:41Z-
Data de disponibilização: dc.date.available2021-10-14T17:58:41Z-
Data de envio: dc.date.issued2010-03-26-
Data de envio: dc.date.issued2010-03-26-
Data de envio: dc.date.issued2003-
Fonte completa do material: dc.identifierhttp://repositorio.unb.br/handle/10482/4025-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/620696-
Descrição: dc.description3 f. : il.-
Descrição: dc.descriptionOBJECTIVE: The aim of this study was to verify whether HDL particles isolated from patients with coronary artery disease (CAD) and low HDL-C had diminished ability to promote cholesterol efflux from cultured cells compared with HDL isolated from subjects without CAD and with normal HDL-C. METHODS: Smooth muscle cells isolated from human aortas cultured and radiolabeled with ³H-cholesterol were loaded with cholesterol and incubated with increasing concentrations of HDL isolated from 13 CAD patients with low HDL-C (CAD group) or from 5 controls without CAD (C group). Efflux of cellular cholesterol was measured by cellular depletion of radiolabeled cholesterol and by the appearance of ³H-cholesterol into experimental medium expressed as a percentage of total labeled cholesterol. RESULTS: Cholesterol efflux increased with the amount of HDL present in the medium, and no difference was found between groups at various HDL protein concentrations: efflux was 28 ± 6.3 percent (C) and 25.5 ± 8.9 percent (CAD) with 25 mg/mL; 34 ± 4.3 percent (C) and 31.9 ± 6.6 percent (CD) with 50 mg/mL and 39.5 ± 3.5 percent (C) and 37.1 ± 4.4 percent (CAD) with 100 mg/mL, HDL. CONCLUSION: Because the HDL fraction of CAD patients with low HDL-C have normal ability to extract cholesterol from cells of the vessel wall, it is suggested that low HDL-C atherogenicity should be ascribed to diminished concentrations of HDL particles rather than to the qualitative properties of the HDL fraction (AU).-
Formato: dc.formatapplication/pdf-
Direitos: dc.rightsAcesso Aberto-
Palavras-chave: dc.subjectCoronariopatias-
Palavras-chave: dc.subjectColesterol-
Palavras-chave: dc.subjectLipoproteínas-
Palavras-chave: dc.subjectArtérias-
Título: dc.titleCellular cholesterol efflux mediated by HDL isolated from subjects with low HDL levels and Coronary artery disease-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional – UNB

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