Ramularia leaf spot : PCR-based methods reveal widespread distribution of Ramulariopsis pseudogycines and limited presence of R. gossypii in Brazil

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorSilva, Aline Suelen-
Autor(es): dc.creatorRenno, Marcelo Henrique Lisboa-
Autor(es): dc.creatorQuitania, Ana Clara Ribeiro-
Autor(es): dc.creatorCafé Filho, Adalberto Correa-
Autor(es): dc.creatorMiller, Robert Neil Gerard-
Autor(es): dc.creatorAraujo, Alderi Emidio-
Autor(es): dc.creatorPinho, Danilo Batista-
Data de aceite: dc.date.accessioned2021-10-14T17:22:16Z-
Data de disponibilização: dc.date.available2021-10-14T17:22:16Z-
Data de envio: dc.date.issued2021-05-10-
Data de envio: dc.date.issued2021-05-10-
Data de envio: dc.date.issued2020-
Fonte completa do material: dc.identifierhttps://repositorio.unb.br/handle/10482/40852-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/606301-
Descrição: dc.descriptionThis preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.-
Descrição: dc.descriptionWhilst Brazil is the fourth largest cotton producer globally, incidence of ramularia leaf spot (RLS) has decreased yield across all growing regions. In 2017-18 and 2018-19 growing seasons, ca. 300 fungal samples were collected from seven Brazilian states. Hyphal tip cultures were obtained for DNA extraction and amplification of partial sequences of the RNA polymerase II gene (RPB2), 28S rRNA, the ribosomal DNA internal transcribed spacers (ITS), actin (ACT), elongation factor (EF1-α) and histone H3 (HIS3) gene regions. Genetic diversity of the isolates was evaluated using fourteen molecular markers. Clade assignments based on the concatenated-sequence tree (RPB2, LSU, EF1-α, ITS, ACT, and HIS3) were identical to those in tree clades generated by RPB2-sequences, as well as in an RPB2 haplotype network, in an ISSR (TGTC)4 dendrogram, and based on morphological comparisons. In total, 252 out of 267 isolates were identified as Ramulariopsis pseudoglycines, indicating this species as the most widespread causal agent of cotton RLS in the Brazilian growing regions today. Validation of the ISSR (TGTC)4 primer as a tool to study the diversity and distribution of Ramulariopsis species will make it possible to carry out extensive RLS sampling studies worldwide.-
Formato: dc.formatapplication/pdf-
Publicador: dc.publisherhttps://doi.org/10.21203/rs.3.rs-477759/v1-
Direitos: dc.rightsAcesso Aberto-
Direitos: dc.rightsThis work is licensed under a CC BY 4.0 License.-
Palavras-chave: dc.subjectAlgodão - pragas e doenças-
Palavras-chave: dc.subjectFungos patogênicos-
Título: dc.titleRamularia leaf spot : PCR-based methods reveal widespread distribution of Ramulariopsis pseudogycines and limited presence of R. gossypii in Brazil-
Tipo de arquivo: dc.typeaula digital-
Aparece nas coleções:Repositório Institucional – UNB

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