Fast determination of benzodiazepines in human urine via liquid-liquid extraction with low temperature partitioning and LC-HRMS.

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorMagalhães, Elisângela Jaqueline-
Autor(es): dc.creatorNascentes, Clésia Cristina-
Autor(es): dc.creatorAugusti, Rodinei-
Autor(es): dc.creatorQueiroz, Maria Eliana Lopes Ribeiro de-
Autor(es): dc.creatorSilva, Júlio César Cardoso da-
Autor(es): dc.creatorAfonso, Robson José de Cássia Franco-
Data de aceite: dc.date.accessioned2019-11-06T13:33:50Z-
Data de disponibilização: dc.date.available2019-11-06T13:33:50Z-
Data de envio: dc.date.issued2015-04-17-
Data de envio: dc.date.issued2015-04-17-
Data de envio: dc.date.issued2012-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/5140-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/558158-
Descrição: dc.descriptionA simple and high-throughput method to simultaneously determine selected benzodiazepines (i.e., diazepam, lorazepam, clonazepam, and bromazepam) in urine was developed and validated. The entire methodology consisted of the applica-tion of an innovative extraction/cleanup procedure, namely liquid-liquid extraction with low-temperature partitioning (LLE-LTP), and analysis by liquid chromatography combined with high-resolution mass spectrometry (LC-HRMS). The LLE-LTP procedure was optimized via factorial design and by evaluating crucial variables, specifically the freez-ing mode (either slow or fast), the urine/acetonitrile volume ratio, and the sample ionic strength. The benzodiazepines were quantified using matrix-matched calibration curves where the following parameters were assessed by validation protocol: in general, linearity range of 17 - 200 μg•L–1 (r > 0.9957); limits of detection lower than 5 μg•L–1; relative standard deviations (RSD) lower than 12.5%; and accuracy ranging from 72.3% to 117%. To test this procedure’s per-formance, the method was applied to determine the content of diazepam in actual urine samples. The validation results obtained for the method demonstrated that the present methodology could be potentially applied in proficient laborato-ries as a routine approach for determining benzodiazepines compounds content in urine.-
Idioma: dc.languageen-
Direitos: dc.rightsThis work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License. Fonte: American Journal of Analytical Chemistry <http://www.scirp.org/journal/PaperInformation.aspx?PaperID=17285#disqusinfor> Acesso em: 05 de fev. 2015.-
Palavras-chave: dc.subjectBenzodiazepines-
Palavras-chave: dc.subjectUrine-
Palavras-chave: dc.subjectExtraction procedure-
Palavras-chave: dc.subjectLow-temperature partitioning-
Título: dc.titleFast determination of benzodiazepines in human urine via liquid-liquid extraction with low temperature partitioning and LC-HRMS.-
Aparece nas coleções:Repositório Institucional - UFOP

Não existem arquivos associados a este item.