Fluorescence spectroscopy of small peptides interacting with microheterogeneous micelles.

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorRomani, Ana Paula-
Autor(es): dc.creatorMarquenzin, Cassia Alessandra-
Autor(es): dc.creatorIto, Amando Siuiti-
Data de aceite: dc.date.accessioned2019-11-06T13:33:08Z-
Data de disponibilização: dc.date.available2019-11-06T13:33:08Z-
Data de envio: dc.date.issued2015-04-01-
Data de envio: dc.date.issued2015-04-01-
Data de envio: dc.date.issued2010-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/4871-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/557890-
Descrição: dc.descriptionMany peptides containing tryptophan have therapeutic uses and can be studied by their fluorescent properties. The biological activity of these peptides involves interactions with many cellular components and micelles can function as carriers inside organisms. We report results from the interaction of small peptides containing tryptophan with several microheterogeneous systems: sodium dodecyl sulphate (SDS) micelles; sodium dodecyl sulphate–poly(ethylene oxide) (SDS–PEO) aggregates; and neutral polymeric micelles. We observed that specific parameters, such as wavelength of maximum emission and fluorescence anisotropy, could be used to ascertain the occurrence of interactions. Affinity constants were determined from changes in the intensity of emission while structural modifications in rotameric conformations were verified from time-resolved measurements. Information about the location and diffusion of peptides in the microheterogeneous systems were obtained from tryptophan emission quenching experiments using N-alkylpyridinium ions. The results show the importance of electrostatic and hydrophobic effects, and of the ionization state of charged residues, in the presence of anionic and amphiphilic SDS in the microheterogeneous systems. Conformational stability of peptides is best preserved in the interaction with the neutral polymeric micelles.-
Idioma: dc.languageen-
Direitos: dc.rightsO Periódico International Journal of Pharmaceutics concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3582470757213.-
Palavras-chave: dc.subjectColloidal carrier-
Palavras-chave: dc.subjectPolymeric micelle-
Palavras-chave: dc.subjectPolymer-surfactant-
Palavras-chave: dc.subjectTryptophan peptides-
Palavras-chave: dc.subjectFluorescence-
Título: dc.titleFluorescence spectroscopy of small peptides interacting with microheterogeneous micelles.-
Aparece nas coleções:Repositório Institucional - UFOP

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