Choline oxidase chemilumin scent assay, after removal of serine from medium, of acetylcholine released in vitro from brain slices.

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorPrado, Marco Antonio Maximo-
Autor(es): dc.creatorSantos, T. Moraes-
Autor(es): dc.creatorFreitas, Renata Nascimento de-
Autor(es): dc.creatorSilva, Marco Aurelio Romano-
Autor(es): dc.creatorGomez, Marcus Vinicius-
Data de aceite: dc.date.accessioned2019-11-06T13:32:55Z-
Data de disponibilização: dc.date.available2019-11-06T13:32:55Z-
Data de envio: dc.date.issued2015-04-01-
Data de envio: dc.date.issued2015-04-01-
Data de envio: dc.date.issued1990-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/4916-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/557791-
Descrição: dc.descriptionA chemiluminescent method has been used recently for the determination of acetylcholine with limitations such as the presence of a cholinesterase inhibitor in the incubation medium, which is indispensable for the study of acetylcholine release by various agents. A modified procedure is presented in which the cholinesterase inhibitor eserine (physostigmine) is extracted from the medium. The results showed complete recovery when labelled acetylcholine was used. This modified procedure was used to determine the release of acetylcholine evoked by tityustoxin and ouabain. The results were comparable to those obtained by bioassay using a strip of guinea pig ileum.-
Idioma: dc.languageen-
Direitos: dc.rightsO Periódico Journal of Neuroscience Methods concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3556580424364.-
Palavras-chave: dc.subjectAcetylcholine assay-
Palavras-chave: dc.subjectChemiluminescent procedure-
Palavras-chave: dc.subjectNeurotransmitter release-
Título: dc.titleCholine oxidase chemilumin scent assay, after removal of serine from medium, of acetylcholine released in vitro from brain slices.-
Aparece nas coleções:Repositório Institucional - UFOP

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