NEDD8 conjugation in Schistosoma mansoni : genome analysis and expression profiles.

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorPereira, Roberta Verciano-
Autor(es): dc.creatorGomes, Matheus de Souza-
Autor(es): dc.creatorOlmo, Roenick Proveti-
Autor(es): dc.creatorSousa, Daniel M.-
Autor(es): dc.creatorPassos, Liana Konovaloff Jannotti-
Autor(es): dc.creatorBabá, Élio Hideo-
Autor(es): dc.creatorBorges, William de Castro-
Autor(es): dc.creatorCota, Renata Guerra de Sá-
Data de aceite: dc.date.accessioned2019-11-06T13:32:47Z-
Data de disponibilização: dc.date.available2019-11-06T13:32:47Z-
Data de envio: dc.date.issued2015-03-16-
Data de envio: dc.date.issued2015-03-16-
Data de envio: dc.date.issued2013-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/4652-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/557733-
Descrição: dc.descriptionNEDD8 is an ubiquitin-like molecule that covalently binds to target proteins through an enzymatic cascade analogous to ubiquitylation. This modifier is known to bind to p53 and p73, as well as all Cullin family proteins, which are essential components of Skp1/Cul-1/F-box protein (SCF)-like Ub ligase complexes. Here, we focused on a genomic analysis of the genes involved in the NEDD8 conjugation pathway in Schistosoma mansoni. The results revealed seven genes related to NEDD8 conjugation that are conserved in Schistosoma japonicum, Caenorhabditis elegans, Drosophila melanogaster and Homo sapiens. We performed quantitative RT-PCR (qRT-PCR), which showed differential profiles for Smnedd8, Smapp1, Smuba3, Smube2f, Smdcn1, Smrbx and Smsenp8 throughout the life cycle of S. mansoni. Upregulation was observed in 3-day-old schistosomula and adult worms for all analysed genes. We also analysed the transcription levels of Cullin family members Smp63 and Smp73, and observed upregulation in early schistosomula, while cercariae and adult worms showed expression levels similar to one another. Taken together, these results suggest that the NEDDylation/ DeNEDDylation pathway controls important cellular regulators during worm development from cercariae to schistosomula and, finally, to adult.-
Idioma: dc.languageen-
Direitos: dc.rightsO Periódico Parasitology International concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3547120211404.-
Palavras-chave: dc.subjectSchistosomula development-
Palavras-chave: dc.subjectDifferential expression-
Título: dc.titleNEDD8 conjugation in Schistosoma mansoni : genome analysis and expression profiles.-
Aparece nas coleções:Repositório Institucional - UFOP

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