Schistosoma mansoni encodes SMT3B and SMT3C molecules responsible for post-translational modification of cellular proteins.

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorCabral, Fernanda Janku-
Autor(es): dc.creatorPereira Junior, Olavo dos Santos-
Autor(es): dc.creatorSilva, Camila Siqueira-
Autor(es): dc.creatorCota, Renata Guerra de Sá-
Autor(es): dc.creatorRodrigues, Vanderlei-
Data de aceite: dc.date.accessioned2019-11-06T13:32:38Z-
Data de disponibilização: dc.date.available2019-11-06T13:32:38Z-
Data de envio: dc.date.issued2015-03-06-
Data de envio: dc.date.issued2015-03-06-
Data de envio: dc.date.issued2008-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/4561-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/557678-
Descrição: dc.descriptionThe sumoylation pathway is a post-translational modification of nuclear proteins widespread among several organisms. SMT3C is the main protein involved in this process and it is covalently conjugated to a diverse assortment of nuclear protein targets. To date, 3 SUMO paralogues (SMT3C, A/B) have been characterized in mammals and plants. In this work we characterized two SUMO related genes, named SMT3B and SMT3C throughout Schistosoma mansoni life cycle. The SmSMTB/C encodes for proteins sharing significant amino acid homology with SMT3. Phylogenetical analyses revealed that both SmSMT3B/C are distinct proteins. Additionally, SmSMT3B and C are expressed in cercariae, adult worms, eggs and schistosomula however SmSMT3C gene showed an expression level 7 to 9 fold higher than SmSMT3B in eggs, schistosomula and adult worms. The comparison between the SmSMT3C genomic and cDNA sequences established that the encoding sequence is interrupted by 3 introns of 70, 37 and 36 bp. Western Blot has shown SMT3 conjugates are present in nuclear and total protein fractions of adults and cercariae. Therefore our results suggest a functional sumoylation pathway, and the presence of two paralogues also suggests the specificity of substrates for SMT3 in S. mansoni.-
Idioma: dc.languageen-
Direitos: dc.rightsO Periódico Parasitology International concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3547120300046.-
Palavras-chave: dc.subjectSchistosoma mansoni-
Palavras-chave: dc.subjectPost-translational modification-
Palavras-chave: dc.subjectSumoylation-
Título: dc.titleSchistosoma mansoni encodes SMT3B and SMT3C molecules responsible for post-translational modification of cellular proteins.-
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