Preliminary analysis of miRNA pathway in Schistosoma mansoni.

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorGomes, Matheus de Souza-
Autor(es): dc.creatorCabral, Fernanda Janku-
Autor(es): dc.creatorPassos, Liana Konovaloff Jannotti-
Autor(es): dc.creatorCarvalho, Omar-
Autor(es): dc.creatorRodrigues, Vanderlei-
Autor(es): dc.creatorBabá, Élio Hideo-
Autor(es): dc.creatorCota, Renata Guerra de Sá-
Data de aceite: dc.date.accessioned2019-11-06T13:32:38Z-
Data de disponibilização: dc.date.available2019-11-06T13:32:38Z-
Data de envio: dc.date.issued2015-03-06-
Data de envio: dc.date.issued2015-03-06-
Data de envio: dc.date.issued2009-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/4552-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/557677-
Descrição: dc.descriptionRNA silencing refers to a series of nuclear and cytoplasmatic processes involved in the post-transcriptional regulation of gene expression or post-transcriptional gene silencing (PTGS), either by sequence-specific mRNA degradation or by translational arrest. The best characterized small RNAs are microRNAs (miRNAs), which predominantly perform gene silencing through post-transcriptional mechanisms. In this work we used bioinformatic approaches to identify the parasitic trematode Schistosoma mansoni sequences that are similar to enzymes involved in the post-transcriptional gene silencing mediated by miRNA pathway.We used amino acid sequences of well-known proteins involved in the miRNA pathway against S. mansoni genome and transcriptome databases identifying a total of 13 putative proteins in the parasite. In addition, the transcript levels of SmDicer1 and SmAgo2/3/4 were identified by qRT-PCR using cercariae, adult worms, eggs and in vitro cultivated schistosomula. Our results showed that the SmDicer1 and SmAgo2/3/4 are differentially expressed during schistosomula development, suggesting that the miRNA pathway is regulated at the transcript level and therefore may control gene expression during the life cycle of S. mansoni.-
Idioma: dc.languageen-
Direitos: dc.rightsO Periódico Parasitology International concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3547120424363.-
Palavras-chave: dc.subjectSchistosoma mansoni-
Palavras-chave: dc.subjectGene expression-
Palavras-chave: dc.subjectPost-transcriptional gene silencing-
Título: dc.titlePreliminary analysis of miRNA pathway in Schistosoma mansoni.-
Aparece nas coleções:Repositório Institucional - UFOP

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