Analysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi.

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorCardoso, Josiane-
Autor(es): dc.creatorLima, Carla de Paula-
Autor(es): dc.creatorLeal, Tiago-
Autor(es): dc.creatorGradia, Daniela Fiori-
Autor(es): dc.creatorFragoso, Stênio Perdigão-
Autor(es): dc.creatorGoldenberg, Samuel-
Autor(es): dc.creatorSá, Renata Guerra-
Autor(es): dc.creatorKrieger, Marco Aurelio-
Data de aceite: dc.date.accessioned2019-11-06T13:32:38Z-
Data de disponibilização: dc.date.available2019-11-06T13:32:38Z-
Data de envio: dc.date.issued2015-03-20-
Data de envio: dc.date.issued2015-03-20-
Data de envio: dc.date.issued2011-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/4715-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/557675-
Descrição: dc.descriptionProteasomes are large protein complexes, whose main function is to degrade unnecessary or damaged proteins. The inhibition of proteasome activity in Trypanosoma cruzi blocks parasite replication and cellular differentiation. We demonstrate that proteasome-dependent proteolysis occurs during the cellular differentiation of T. cruzi from replicative non-infectious epimastigotes to non-replicative and infectious trypomastigotes (metacyclogenesis). No peaks of ubiquitinmediated degradation were observed and the profile of ubiquitinated conjugates was similar at all stages of differentiation. However, an analysis of carbonylated proteins showed significant variation in oxidized protein levels at the various stages of differentiation and the proteasome inhibition also increased oxidized protein levels. Our data suggest that different proteasome complexes coexist during metacyclogenesis. The 20S proteasome may be free or linked to regulatory particles (PA700, PA26 and PA200), at specific cell sites and the coordinated action of these complexes would make it possible for proteolysis of ubiquitin-tagged proteins and oxidized proteins, to coexist in the cell.-
Idioma: dc.languageen-
Direitos: dc.rightsNenhuma autorização é necessária para depósito dos artigos publicados pelos Periódicos PloS em repositório. Fonte: Plos <http://www.plos.org/open-access/> Acesso em: 14 nov. 2014.-
Título: dc.titleAnalysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi.-
Aparece nas coleções:Repositório Institucional - UFOP

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