Atenção: Todas as denúncias são sigilosas e sua identidade será preservada.
Os campos nome e e-mail são de preenchimento opcional
Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.creator | Santos, Ramon de Freitas | - |
Autor(es): dc.creator | Pôssa, Marcela Auxiliadora Souza | - |
Autor(es): dc.creator | Bastos, Matheus Silva e | - |
Autor(es): dc.creator | Guedes, Paulo Marcos da Matta | - |
Autor(es): dc.creator | Almeida, Márcia Rogéria de | - |
Autor(es): dc.creator | DeMarco, Ricardo | - |
Autor(es): dc.creator | Almeida, Sergio Verjovski | - |
Autor(es): dc.creator | Bahia, Maria Terezinha | - |
Autor(es): dc.creator | Fietto, Juliana Lopes Rangel | - |
Data de aceite: dc.date.accessioned | 2019-11-06T13:32:34Z | - |
Data de disponibilização: dc.date.available | 2019-11-06T13:32:34Z | - |
Data de envio: dc.date.issued | 2015-03-12 | - |
Data de envio: dc.date.issued | 2015-03-12 | - |
Data de envio: dc.date.issued | 2009 | - |
Fonte completa do material: dc.identifier | http://www.repositorio.ufop.br/handle/123456789/4609 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/capes/557650 | - |
Descrição: dc.description | Background: The protozoan Trypanosoma cruzi is the causative agent of Chagas disease. There are no vaccines or effective treatment, especially in the chronic phase when most patients are diagnosed. There is a clear necessity to develop new drugs and strategies for the control and treatment of Chagas disease. Recent papers have suggested the ecto-nucleotidases (from CD39 family) from pathogenic agents as important virulence factors. In this study we evaluated the influence of Ecto- Nucleoside-Triphosphate-Diphosphohydrolase (Ecto-NTPDase) activity on infectivity and virulence of T. cruzi using both in vivo and in vitro models. Methodology/Principal Findings: We followed Ecto-NTPDase activities of Y strain infective forms (trypomastigotes) obtained during sequential sub-cultivation in mammalian cells. ATPase/ADPase activity ratios of cell-derived trypomastigotes decreased 3- to 6-fold and infectivity was substantially reduced during sequential sub-cultivation. Surprisingly, at third to fourth passages most of the cell-derived trypomastigotes could not penetrate mammalian cells and had differentiated into amastigote-like parasites that exhibited 3- to 4-fold lower levels of Ecto-NTPDase activities. To evidence the participation of T. cruzi Ecto-NTPDase1 in the infective process, we evaluated the effect of known Ecto- ATPDase inhibitors (ARL 67156, Gadolinium and Suramin), or anti-NTPDase-1 polyclonal antiserum on ATPase and ADPase hydrolytic activities in recombinant T. cruzi NTPDase-1 and in live trypomastigotes. All tests showed a partial inhibition of Ecto-ATPDase activities and a marked inhibition of trypomastigotes infectivity. Mice infections with Ecto-NTPDase-inhibited trypomastigotes produced lower levels of parasitemia and higher host survival than with non-inhibited control parasites. Conclusions/Significance: Our results suggest that Ecto-ATPDases act as facilitators of infection and virulence in vitro and in vivo and emerge as target candidates in chemotherapy of Chagas disease. | - |
Idioma: dc.language | en | - |
Direitos: dc.rights | Nenhuma autorização é necessária para depósito dos artigos publicados pelos Periódicos PloS em repositório. Fonte: Plos <http://www.plos.org/open-access/> Acesso em: 14 nov. 2014. | - |
Título: dc.title | Influence of ecto-nucleoside triphosphate diphosphohydrolase activity on trypanosoma cruzi infectivity and virulence. | - |
Aparece nas coleções: | Repositório Institucional - UFOP |
O Portal eduCAPES é oferecido ao usuário, condicionado à aceitação dos termos, condições e avisos contidos aqui e sem modificações. A CAPES poderá modificar o conteúdo ou formato deste site ou acabar com a sua operação ou suas ferramentas a seu critério único e sem aviso prévio. Ao acessar este portal, você, usuário pessoa física ou jurídica, se declara compreender e aceitar as condições aqui estabelecidas, da seguinte forma: