Ecto-nucleotidase activities of promastigotes from leishmania (Viannia) braziliensis relates to parasite infectivity and disease clinical outcome.

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Autor(es): dc.creatorLeite, Pauline Martins-
Autor(es): dc.creatorGomes, Rodrigo Saar-
Autor(es): dc.creatorFigueiredo, Amanda Braga de-
Autor(es): dc.creatorSerafim, Tiago Donatelli-
Autor(es): dc.creatorTafuri, Wagner Luiz-
Autor(es): dc.creatorGomes, Carolina Cavaliéri-
Autor(es): dc.creatorMoura, Sandra Aparecida de Lima-
Autor(es): dc.creatorFietto, Juliana Lopes Rangel-
Autor(es): dc.creatorMelo, Maria Norma-
Autor(es): dc.creatorDias, Fátima Ribeiro-
Autor(es): dc.creatorOliveira, Milton Adriano Pelli de-
Autor(es): dc.creatorRabello, Ana-
Autor(es): dc.creatorAfonso, Luís Carlos Crocco-
Data de aceite: dc.date.accessioned2019-11-06T13:32:27Z-
Data de disponibilização: dc.date.available2019-11-06T13:32:27Z-
Data de envio: dc.date.issued2015-02-27-
Data de envio: dc.date.issued2015-02-27-
Data de envio: dc.date.issued2012-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/4517-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/557602-
Descrição: dc.descriptionBackground: Leishmania (Viannia) braziliensis has been associated with a broad range of clinical manifestations ranging from a simple cutaneous ulcer to destructive mucosal lesions. Factors leading to this diversity of clinical presentations are not clear, but parasite factors have lately been recognized as important in determining disease progression. Given the fact that the activity of ecto-nucleotidases correlates with parasitism and the development of infection, we evaluated the activity of these enzymes in promastigotes from 23 L. braziliensis isolates as a possible parasite-related factor that could influence the clinical outcome of the disease. Methodology/Principal Findings: Our results show that the isolates differ in their ability to hydrolyze adenine nucleotides. Furthermore, we observed a positive correlation between the time for peak of lesion development in C57BL/6J mice and enzymatic activity and clinical manifestation of the isolate. In addition, we found that L. (V.) braziliensis isolates obtained from mucosal lesions hydrolyze higher amounts of adenine nucleotides than isolates obtained from skin lesions. One isolate with high (PPS6m) and another with low (SSF) ecto-nucleotidase activity were chosen for further studies. Mice inoculated with PPS6m show delayed lesion development and present larger parasite loads than animals inoculated with the SSF isolate. In addition, PPS6m modulates the host immune response by inhibiting dendritic cell activation and NO production by activated J774 macrophages. Finally, we observed that the amastigote forms from PPS6m and SSF isolates present low enzymatic activity that does not interfere with NO production and parasite survival in macrophages. Conclusions/Significance: Our data suggest that ecto-nucleotidases present on the promastigote forms of the parasite may interfere with the establishment of the immune response with consequent impaired ability to control parasite dissemination and this may be an important factor in determining the clinical outcome of leishmaniasis.-
Idioma: dc.languageen-
Direitos: dc.rightsNenhuma autorização é necessária para depósito dos artigos publicados pelos Periódicos PloS em repositório. Fonte: Plos <http://www.plos.org/open-access/> Acesso em 14 nov. 2014.-
Palavras-chave: dc.subjectLeishmania braziliensis-
Título: dc.titleEcto-nucleotidase activities of promastigotes from leishmania (Viannia) braziliensis relates to parasite infectivity and disease clinical outcome.-
Aparece nas coleções:Repositório Institucional - UFOP

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