Performance of LBSap vaccine after intradermal challenge with L. infantum and saliva of Lu. longipalpis : immunogenicity and parasitological evaluation.

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorRoatt, Bruno Mendes-
Autor(es): dc.creatorSoares, Rodrigo Dian de Oliveira Aguiar-
Autor(es): dc.creatorSouza, Juliana Vitoriano de-
Autor(es): dc.creatorVital, Wendel Coura-
Autor(es): dc.creatorBraga, Samuel Leôncio-
Autor(es): dc.creatorOliveira, Rodrigo Corrêa de-
Autor(es): dc.creatorMartins Filho, Olindo Assis-
Autor(es): dc.creatorCarvalho, Andréa Teixeira de-
Autor(es): dc.creatorLana, Marta de-
Autor(es): dc.creatorGontijo, Nelder Figueiredo-
Autor(es): dc.creatorMarques, Marcos José-
Autor(es): dc.creatorGiunchetti, Rodolfo Cordeiro-
Autor(es): dc.creatorReis, Alexandre Barbosa-
Data de aceite: dc.date.accessioned2019-11-06T13:31:57Z-
Data de disponibilização: dc.date.available2019-11-06T13:31:57Z-
Data de envio: dc.date.issued2015-01-22-
Data de envio: dc.date.issued2015-01-22-
Data de envio: dc.date.issued2012-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/4329-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/557392-
Descrição: dc.descriptionIn the last decade, the search for new vaccines against canine visceral leishmaniasis has intensified. However, the pattern related to immune protection during long periods after experimental infection in vaccine trials is still not fully understood. Herein, we investigated the immunogenicity and parasitological levels after intradermal challenge with Leishmania infantum plus salivary gland extract in dogs immunized with a vaccine composed of L. braziliensis antigens plus saponin as an adjuvant (LBSap vaccine). The LBSap vaccine elicited higher levels of total anti-Leishmania IgG as well as both IgG1 and IgG2. Furthermore, dogs vaccinated had increased levels of lymphocytes, particularly circulating B cells (CD21+) and both CD4+ and CD8+ T lymphocytes. LBSap also elicited an intense in vitro cell proliferation associated with higher levels of CD4+ T lymphocytes specific for vaccine soluble antigen and soluble lysate of L. infantum antigen even 885 days after experimental challenge. Furthermore, LBSap vaccinated dogs presented high IFN-c and low IL-10 and TGF-b1 expression in spleen with significant reduction of parasite load in this tissue. Overall, our results validate the potential of LBSap vaccine to protect against L. infantum experimental infection and strongly support further evaluation of efficiency of LBSap against CVL in natural infection conditions.-
Idioma: dc.languageen-
Direitos: dc.rightsNenhuma autorização é necessária para depósito em repositório dos artigos publicados pelos Periódicos PloS . Fonte: <!--td {border: 1px solid #ccc;}br {mso-data-placement:same-cell;}-->. Acesso em: 12 fev. 2014.-
Palavras-chave: dc.subjectLeishmania infantum-
Título: dc.titlePerformance of LBSap vaccine after intradermal challenge with L. infantum and saliva of Lu. longipalpis : immunogenicity and parasitological evaluation.-
Aparece nas coleções:Repositório Institucional - UFOP

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