Cytokine and nitric oxide patterns in dogs immunized with LBSap vaccine, before and after experimental challenge with Leishmania chagasi plus saliva of Lutzomyia longipalpis.

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorResende, Lucilene Aparecida-
Autor(es): dc.creatorRoatt, Bruno Mendes-
Autor(es): dc.creatorSoares, Rodrigo Dian de Oliveira Aguiar-
Autor(es): dc.creatorViana, Kelvison Fernandes-
Autor(es): dc.creatorMendonça, Ludmila Zanandreis de-
Autor(es): dc.creatorLanna, Mariana Ferreira-
Autor(es): dc.creatorLemos, Denise da Silveira-
Autor(es): dc.creatorOliveira, Rodrigo Corrêa de-
Autor(es): dc.creatorMartins Filho, Olindo Assis-
Autor(es): dc.creatorFujiwara, Ricardo Toshio-
Autor(es): dc.creatorCarneiro, Cláudia Martins-
Autor(es): dc.creatorReis, Alexandre Barbosa-
Autor(es): dc.creatorGiunchetti, Rodolfo Cordeiro-
Data de aceite: dc.date.accessioned2019-11-06T13:31:56Z-
Data de disponibilização: dc.date.available2019-11-06T13:31:56Z-
Data de envio: dc.date.issued2015-01-22-
Data de envio: dc.date.issued2015-01-22-
Data de envio: dc.date.issued2013-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/4330-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/557384-
Descrição: dc.descriptionIn the studies presented here, dogs were vaccinated against Leishmania (Leishmania) cha-gasi challenge infection using a preparation of Leishmania braziliensis promastigote proteinsand saponin as adjuvant (LBSap). Vaccination with LBSap induced a prominent type 1immune response that was characterized by increased levels of interleukin (IL-) 12 andinterferon gamma (IFN- _) production by peripheral blood mononuclear cells (PBMC) uponstimulation with soluble vaccine antigen. Importantly, results showed that this type ofresponsiveness was sustained after challenge infection; at day 90 and 885 after L. chagasichallenge infection, PBMCs from LBSap vaccinated dogs produced more IL-12, IFN- _ andconcomitant nitric oxide (NO) when stimulated with Leishmania antigens as comparedto PBMCs from respective control groups (saponin, LB- treated, or non-treated controldogs). Moreover, transforming growth factor (TGF)- _ decreased in the supernatant of SLcA-stimulated PBMCs in the LBSap group at 90 days. Bone marrow parasitological analysisrevealed decreased frequency of parasitism in the presence of vaccine antigen. It is con-cluded that vaccination of dogs with LBSap vaccine induced a long-lasting type 1 immuneresponse against L. chagasi challenge infection.-
Idioma: dc.languageen-
Direitos: dc.rightsO Periódico Veterinary Parasitology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3440300508466.-
Palavras-chave: dc.subjectVaccine-
Palavras-chave: dc.subjectCanine visceral leishmaniasis-
Palavras-chave: dc.subjectCytokines-
Palavras-chave: dc.subjectimmunoprotection-
Palavras-chave: dc.subjectLeishmania chagasi-
Título: dc.titleCytokine and nitric oxide patterns in dogs immunized with LBSap vaccine, before and after experimental challenge with Leishmania chagasi plus saliva of Lutzomyia longipalpis.-
Aparece nas coleções:Repositório Institucional - UFOP

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