Innovations in diagnosis and post-therapeutic monitoring of Chagas disease: simultaneous flow cytometric detection of IgG1 antibodies anti-live amastigote, anti-live trypomastigote, and anti-fixed epimastigote forms of Trypanosoma cruzi.

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorAlessio, Glaucia Diniz-
Autor(es): dc.creatorCôrtes, Denise Fonseca-
Autor(es): dc.creatorAssis, Girley Francisco Machado de-
Autor(es): dc.creatorSales Júnior, Policarpo Ademar-
Autor(es): dc.creatorFerro, Eloisa Amália Vieira-
Autor(es): dc.creatorAntonelli, Lis Ribeiro do Vale-
Autor(es): dc.creatorCarvalho, Andréa Teixeira de-
Autor(es): dc.creatorMartins Filho, Olindo Assis-
Autor(es): dc.creatorLana, Marta de-
Data de aceite: dc.date.accessioned2019-11-06T13:31:18Z-
Data de disponibilização: dc.date.available2019-11-06T13:31:18Z-
Data de envio: dc.date.issued2014-11-19-
Data de envio: dc.date.issued2014-11-19-
Data de envio: dc.date.issued2014-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/3960-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/557122-
Descrição: dc.descriptionThis study developed a remarkable methodological innovation (FC-ATE) which enables simultaneous detection of antibodies specific to the three evolutive forms of Trypanosoma cruzi: live amastigote (AMA), live trypomastigote (TRYPO), and fixed epimastigote (EPI) using a differential fluorescence staining as low (AMA), intermediate (TRYPO), and high (EPI). An outstanding performance (100%) was observed in the discrimination of the chagasic (CH) and non-chagasic (NCH) patients. In the applicability of FC-ATE in the diagnosis of Chagas disease, 100% of the CH samples presented positivity in the percentage of positive fluorescent parasites (PPFP) for all the three forms of T. cruzi. Moreover, 94% of the samples of NCH presented negative values of PPFP with AMA and TRYPO, and 88% with EPI. Samples from the NCH group with falsepositive results were those belonging to the leishmaniasis patients. Considering the applicability of this technique in post-therapeuticmonitoring of Chagas disease, 100% of non-treated (NT) and treated non-cured (TNC) samples were positive with the three T. cruzi evolutive forms, while a percentage of 100% fromsamples of the treated cured (TC) patientswere negativewith AMA, 93% with TRYPO and 96% with EPI. The comparison between FC-ATE and two other flow cytometric tests using the same samples of patients NT, TNC and TC showed that the three techniques presented different reactivities, although categorical correlation between the methodologies was observed. Taken together, the results obtained with the novel FC-ATE method have shown an outstanding performance in the diagnosis and post-therapeutic monitoring of Chagas disease.-
Idioma: dc.languageen-
Direitos: dc.rightsO Periódico Journal of Immunological Methods concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3483170605107.-
Palavras-chave: dc.subjectChagas disease-
Palavras-chave: dc.subjectFlow cytometry-
Palavras-chave: dc.subjectSerology-
Palavras-chave: dc.subjectDiagnosis-
Título: dc.titleInnovations in diagnosis and post-therapeutic monitoring of Chagas disease: simultaneous flow cytometric detection of IgG1 antibodies anti-live amastigote, anti-live trypomastigote, and anti-fixed epimastigote forms of Trypanosoma cruzi.-
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