Chloroaluminium phthalocyanine polymeric nanoparticles as photosensitisers: photophysical and physicochemical characterisation, release and phototoxicity in vitro.

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorPaula, Carina Silva de-
Autor(es): dc.creatorTedesco, Antônio Cláudio-
Autor(es): dc.creatorLucas Primo, Fernando-
Autor(es): dc.creatorVilela, José Mário Carneiro-
Autor(es): dc.creatorAndrade, Margareth Spangler-
Autor(es): dc.creatorMosqueira, Vanessa Carla Furtado-
Data de aceite: dc.date.accessioned2019-11-06T13:30:48Z-
Data de disponibilização: dc.date.available2019-11-06T13:30:48Z-
Data de envio: dc.date.issued2014-11-10-
Data de envio: dc.date.issued2014-11-10-
Data de envio: dc.date.issued2013-
Fonte completa do material: dc.identifierhttp://www.repositorio.ufop.br/handle/123456789/3752-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/556916-
Descrição: dc.descriptionABSTRACT: Nanoparticles of poly(D,L-lactide-co-glycolide), poly(D,L-lactide) and polyethylene glycol-blockpoly (D,L-lactide) were developed to encapsulate chloroaluminium phthalocyanine (AlClPc), a new hydrophobic photosensitiser used in photodynamic therapy (PDT). The mean nanoparticle size varied from 115 to 274 nm, and the encapsulation efficiency ranged from 57% to 96% due to drug precipitation induced by different types of polymer. All nanoparticle formulations presented negative zeta potential values (–37 mV to –59 mV), explaining their colloidal stability. The characteristic photophysical parameters were analysed: the absorption spectrum profile, fluorescence quantum yield and transient absorbance decay, with similar values for free and nanoparticles of AlClPc. The time-resolved spectroscopy measurements for AlClPc triplet excited state lifetimes indicate that encapsulation in nanocapsules increases triplet lifetime, which is advantageous for PDT efficiency. A sustained release profile over 168 h was obtained using external sink method. An in vitro phototoxic effect higher than 80% was observed in human fibroblasts at low laser light doses (3 J/cm2) with 10 lM of AlClPc. The AlClPc loaded within polymeric nanocapsules presented suitable physical stability, improved photophysical properties, sustained released profile and suitable activity in vitro to be considered a promising formulation for PDT.-
Idioma: dc.languageen-
Direitos: dc.rightsO Periódico European Journal of Pharmaceutical Sciencesconcede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3457171366694.-
Palavras-chave: dc.subjectPhotodynamic therapy-
Palavras-chave: dc.subjectPolymeric nanoparticles-
Palavras-chave: dc.subjectChloroaluminium phthalocyanine-
Palavras-chave: dc.subjectRelease kinetic-
Título: dc.titleChloroaluminium phthalocyanine polymeric nanoparticles as photosensitisers: photophysical and physicochemical characterisation, release and phototoxicity in vitro.-
Aparece nas coleções:Repositório Institucional - UFOP

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