Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts.

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorSerra, Carla Penido-
Autor(es): dc.creatorCôrtes, Steyner de França-
Autor(es): dc.creatorLombardi, Júlio Antônio-
Autor(es): dc.creatorOliveira, Alaíde Braga de-
Autor(es): dc.creatorBraga, Fernão Castro-
Data de aceite: dc.date.accessioned2019-11-06T13:24:35Z-
Data de disponibilização: dc.date.available2019-11-06T13:24:35Z-
Data de envio: dc.date.issued2012-07-30-
Data de envio: dc.date.issued2012-07-30-
Data de envio: dc.date.issued2005-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/123456789/1215-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/554776-
Descrição: dc.descriptionAnew method for the in vitro screening of plant extracts with potential angiotensin-converting enzyme (ACE) inhibitory activity is proposed. The method is based on the cleavage of the substrate hippuryl-glycyl-glycine by ACE and subsequent reaction with trinitrobenzenesulfonic acid to form 2,4,6-trinitrophenyl-glycyl-glycine, whose absorbance is determined at 415nm in a microtitre plate reader. Rabbit lung dehydrated by acetone was employed as an enzyme source. Validation of the method showed satisfactory intra-day (CV ¼ 7.63%) and inter-day precision (CV ¼ 13.61%), recovery (97–102.1%), sensitivity (IC50 ¼ 14.1 nmol/l) and linearity in the range 7.5–120 mmol/l of glycyl-glycine (r2 ¼ 0:9921). Besides, the method showed good correlation with a HPLC assay already established for the screening of ACE inhibitors (r ¼ 0:9935 and 0:9034; respectively, for captopril solutions and for plant extracts). The method involves only inexpensive reagents and apparatus.-
Idioma: dc.languageen-
Direitos: dc.rightsO periódico Phytomedicine concede permissão ao repositório Instucional da UFOP para depositar este artigo. Número da licença: 3233050124767.-
Palavras-chave: dc.subjectIn vitro colorimetric assay-
Palavras-chave: dc.subjectPlant extracts-
Palavras-chave: dc.subjectScreening-
Palavras-chave: dc.subjectAngiotensin-converting enzyme inhibitors-
Título: dc.titleValidation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts.-
Aparece nas coleções:Repositório Institucional - UFOP

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