The PLC1 encoded phospholipase C in the yeast Saccharomyces cerevisiae is essential for glucose-induced phosphatidylinositol turnover and activation of plasma membrane H -ATPase

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorCoccetti, Paola-
Autor(es): dc.creatorTisi, Renata-
Autor(es): dc.creatorMartegani, Enzo-
Autor(es): dc.creatorTeixeira, Leonardo Souza-
Autor(es): dc.creatorBrandão, Rogélio Lopes-
Autor(es): dc.creatorCastro, Ieso de Miranda-
Autor(es): dc.creatorThevelein, Johan Maria-
Data de aceite: dc.date.accessioned2019-11-06T13:24:30Z-
Data de disponibilização: dc.date.available2019-11-06T13:24:30Z-
Data de envio: dc.date.issued2012-07-19-
Data de envio: dc.date.issued2012-07-19-
Data de envio: dc.date.issued1998-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/123456789/1179-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/554744-
Descrição: dc.descriptionAddition of glucose to glucose-deprived cells of the yeast Saccharomyces cerevisiae triggers rapid turnover of phosphatidylinositol, phosphatidylinositol-phosphate and phosphatidylinositol 4,5-bisphosphate. Glucose stimulation of PI turnover was measured both as an increase in the specific ratio of 32P-labeling and as an increase in the level of diacylglycerol after addition of glucose. Glucose also causes rapid activation of plasma membrane H.-ATPase. We show that in a mutant lacking the PLC1 encoded phospholipase C, both processes were strongly reduced. Compound 48/80, a known inhibitor of mammalian phospholipase C, inhibits both processes. However, activation of the plasma membrane H.- ATPase is only inhibited by concentrations of compound 48/80 that strongly inhibit phospholipid turnover. Growth was inhibited by even lower concentrations. Our data suggest that in yeast cells, glucose triggers through activation of the PLC1 gene product a signaling pathway initiated by phosphatidylinositol turnover and involved in activation of the plasma membrane H.-ATPase.-
Idioma: dc.languageen-
Direitos: dc.rightsO Periódico Biochimica et Biophysica Acta. Molecular Cell Research concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3266591238516-
Palavras-chave: dc.subjectCompound 48/80-
Palavras-chave: dc.subjectSaccharomyces cerevisiae-
Palavras-chave: dc.subjectGlucose induction-
Palavras-chave: dc.subjectSignal transduction-
Palavras-chave: dc.subjectMedium acidification-
Título: dc.titleThe PLC1 encoded phospholipase C in the yeast Saccharomyces cerevisiae is essential for glucose-induced phosphatidylinositol turnover and activation of plasma membrane H -ATPase-
Aparece nas coleções:Repositório Institucional - UFOP

Não existem arquivos associados a este item.