Clinical value of anti-Leishmania (Leishmania) chagasi IgG titers detected by flow cytometry to distinguish infected from vaccinated dogs.

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorAndrade, Renata Aline de-
Autor(es): dc.creatorReis, Alexandre Barbosa-
Autor(es): dc.creatorGontijo, Célia Maria Ferreira-
Autor(es): dc.creatorBraga, Lidiane Bento-
Autor(es): dc.creatorRocha, Roberta Dias Rodrigues-
Autor(es): dc.creatorAraújo, Márcio Sobreira Silva-
Autor(es): dc.creatorVianna, Leonardo Rocha-
Autor(es): dc.creatorMartins Filho, Olindo Assis-
Data de aceite: dc.date.accessioned2019-11-06T13:24:13Z-
Data de disponibilização: dc.date.available2019-11-06T13:24:13Z-
Data de envio: dc.date.issued2012-07-10-
Data de envio: dc.date.issued2012-07-10-
Data de envio: dc.date.issued2007-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/123456789/1032-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/554654-
Descrição: dc.descriptionLeishmune® vaccination covers a broader number of endemic areas of canine visceral leishmaniasis (CVL) and therefore the development of new serological devices able to discriminate CVL from Leishmune® vaccinees becomes an urgent need considering the post-vaccine seroconversion detected throughout conventional methodologies. Herein, we have described the establishment of a flow cytometry based methodology to detect anti-fixedL. ( L. ) chagasipromastigotes antibodies (FC-AFPA-IgG, FC-AFPA-IgG1 and FC-AFPA-IgG2) in sera samples from Leishmania ( Leishmania ) chagasiinfected dogs and Leishmune® vaccinees. The results of FC-AFPA were reported along the sera titration curve (1:128–1:524,288), as percentage-of-positive-fluorescent-parasite (PPFP). The use of PPFP = 20% as a cut-off edge to segregate negative and positive results at sera dilution 1:2048 revealed outstanding performance indexes that elect FC-AFPA-IgG and IgG2 (both detected by polyclonal FITC-labeled second step reagent) applicable to the serological diagnosis of CVL, with 100% of specificity for both IgG and IgG2 and 97 and 93% of sensitivity, respectively. Moreover, FC-AFPA-IgG, applied at sera dilution 1:2048, also appeared as a useful tool to discriminate L. chagasiinfected dogs from Leishmune® vaccinees, with 76% of specificity. Outstanding likelihood indexes further support the performance of FC-AFPA-IgG for exclusion diagnosis of CVL in Leishmune® vaccinees. Analysis of FC-AFPA-IgG at sera dilution 1:8192 revealed the most outstanding indexes, demonstrating that besides the ability of PPFP 20% to exclude the diagnosis of CVL, a PPFP values higher 80%, mostly observed for infected dogs (INF) have a minimal change to come from a non-infected animal (NI) or Leishmune®.-
Idioma: dc.languageen-
Direitos: dc.rightsO Periódico Veterinary Immunology and Immunopathology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3291410040834-
Palavras-chave: dc.subjectLeishmania chagasi-
Palavras-chave: dc.subjectFlow cytometry-
Palavras-chave: dc.subjectCanine visceral leishmaniasis-
Palavras-chave: dc.subjectvaccine-
Título: dc.titleClinical value of anti-Leishmania (Leishmania) chagasi IgG titers detected by flow cytometry to distinguish infected from vaccinated dogs.-
Aparece nas coleções:Repositório Institucional - UFOP

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