Atenção: Todas as denúncias são sigilosas e sua identidade será preservada.
Os campos nome e e-mail são de preenchimento opcional
Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.creator | Andrade, Renata Aline de | - |
Autor(es): dc.creator | Reis, Alexandre Barbosa | - |
Autor(es): dc.creator | Gontijo, Célia Maria Ferreira | - |
Autor(es): dc.creator | Braga, Lidiane Bento | - |
Autor(es): dc.creator | Rocha, Roberta Dias Rodrigues | - |
Autor(es): dc.creator | Araújo, Márcio Sobreira Silva | - |
Autor(es): dc.creator | Vianna, Leonardo Rocha | - |
Autor(es): dc.creator | Martins Filho, Olindo Assis | - |
Data de aceite: dc.date.accessioned | 2019-11-06T13:24:13Z | - |
Data de disponibilização: dc.date.available | 2019-11-06T13:24:13Z | - |
Data de envio: dc.date.issued | 2012-07-10 | - |
Data de envio: dc.date.issued | 2012-07-10 | - |
Data de envio: dc.date.issued | 2007 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/123456789/1032 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/capes/554654 | - |
Descrição: dc.description | Leishmune® vaccination covers a broader number of endemic areas of canine visceral leishmaniasis (CVL) and therefore the development of new serological devices able to discriminate CVL from Leishmune® vaccinees becomes an urgent need considering the post-vaccine seroconversion detected throughout conventional methodologies. Herein, we have described the establishment of a flow cytometry based methodology to detect anti-fixedL. ( L. ) chagasipromastigotes antibodies (FC-AFPA-IgG, FC-AFPA-IgG1 and FC-AFPA-IgG2) in sera samples from Leishmania ( Leishmania ) chagasiinfected dogs and Leishmune® vaccinees. The results of FC-AFPA were reported along the sera titration curve (1:128–1:524,288), as percentage-of-positive-fluorescent-parasite (PPFP). The use of PPFP = 20% as a cut-off edge to segregate negative and positive results at sera dilution 1:2048 revealed outstanding performance indexes that elect FC-AFPA-IgG and IgG2 (both detected by polyclonal FITC-labeled second step reagent) applicable to the serological diagnosis of CVL, with 100% of specificity for both IgG and IgG2 and 97 and 93% of sensitivity, respectively. Moreover, FC-AFPA-IgG, applied at sera dilution 1:2048, also appeared as a useful tool to discriminate L. chagasiinfected dogs from Leishmune® vaccinees, with 76% of specificity. Outstanding likelihood indexes further support the performance of FC-AFPA-IgG for exclusion diagnosis of CVL in Leishmune® vaccinees. Analysis of FC-AFPA-IgG at sera dilution 1:8192 revealed the most outstanding indexes, demonstrating that besides the ability of PPFP 20% to exclude the diagnosis of CVL, a PPFP values higher 80%, mostly observed for infected dogs (INF) have a minimal change to come from a non-infected animal (NI) or Leishmune®. | - |
Idioma: dc.language | en | - |
Direitos: dc.rights | O Periódico Veterinary Immunology and Immunopathology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3291410040834 | - |
Palavras-chave: dc.subject | Leishmania chagasi | - |
Palavras-chave: dc.subject | Flow cytometry | - |
Palavras-chave: dc.subject | Canine visceral leishmaniasis | - |
Palavras-chave: dc.subject | vaccine | - |
Título: dc.title | Clinical value of anti-Leishmania (Leishmania) chagasi IgG titers detected by flow cytometry to distinguish infected from vaccinated dogs. | - |
Aparece nas coleções: | Repositório Institucional - UFOP |
O Portal eduCAPES é oferecido ao usuário, condicionado à aceitação dos termos, condições e avisos contidos aqui e sem modificações. A CAPES poderá modificar o conteúdo ou formato deste site ou acabar com a sua operação ou suas ferramentas a seu critério único e sem aviso prévio. Ao acessar este portal, você, usuário pessoa física ou jurídica, se declara compreender e aceitar as condições aqui estabelecidas, da seguinte forma: