Analysis of the cytokine profile in spleen cells from dogs naturally infected by Leishmania chagasi.

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorLage, Ramon Silva-
Autor(es): dc.creatorOliveira, Guilherme Corrêa de-
Autor(es): dc.creatorBusek, Solange Cristina Uber-
Autor(es): dc.creatorGuerra, Luanda Liboreiro-
Autor(es): dc.creatorGiunchetti, Rodolfo Cordeiro-
Autor(es): dc.creatorOliveira, Rodrigo Corrêa de-
Autor(es): dc.creatorReis, Alexandre Barbosa-
Data de aceite: dc.date.accessioned2019-11-06T13:24:13Z-
Data de disponibilização: dc.date.available2019-11-06T13:24:13Z-
Data de envio: dc.date.issued2012-07-10-
Data de envio: dc.date.issued2012-07-10-
Data de envio: dc.date.issued2007-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/123456789/1027-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/554652-
Descrição: dc.descriptionRecent studies suggest that asymptomatic dogs infected with canine visceral leishmaniasis (CVL) develop a Th1 immunological profile whilst oligosymptomatic and symptomatic CVL-infected animals present a Th2 profile. In the present study, an RT-PCR method has been standardised and employed to evaluate the frequency and the semi-quantitative level of expression of the cytokines IL-4, IL-10, IL-12, INF- g and TNF- a in splenocytes of 30 dogs naturally infected with Leishmania chagasiand of 7 non-infected dogs (NID). An increase in the level of expression of IL-12 ( p = 0.059) was detected in all CVL-infected dogs compared with NID. In dogs exhibiting high parasitism, the frequency of expression of IL-10 was higher ( p = 0.011) than in animals presenting low parasitism or medium parasitism (MP) and in NID animals, whilst the level of expression of IL-10 was higher (p = 0.0094) than in animals exhibiting MP and in the NID group. Positive correlations between the levels of expression of IL-10 with respect to the progression of the disease (IL-10: r = 0.3510; p = 0.0337) and the levels of expression of IL-10 and INF- g increase in parasitism (IL-10: r = 0.3428; p = 0.0438 and INF- g: r = 0.4690; p = 0.0045) were observed. Such data suggest that CVL is marked by a balanced production of Th1 and Th2 cytokines, with a predominant accumulation of IL-10 as a consequence of an increase in parasitic load and progression of the disease, and INF- g was related with the increase in parasitic load.-
Idioma: dc.languageen-
Direitos: dc.rightsO Periódico Veterinary Immunology and Immunopathology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3303250782673-
Palavras-chave: dc.subjectCytokines-
Palavras-chave: dc.subjectRT-PCR-
Palavras-chave: dc.subjectCanine disease-
Palavras-chave: dc.subjectVisceral leishmaniasis-
Palavras-chave: dc.subjectLeishmania chagasi-
Título: dc.titleAnalysis of the cytokine profile in spleen cells from dogs naturally infected by Leishmania chagasi.-
Aparece nas coleções:Repositório Institucional - UFOP

Não existem arquivos associados a este item.