Ring coupler moving via a biased-diffusion mechanism [Microtubule depolymerization as a biological machine]

Registro completo de metadados
Autor(es): dc.creatorEfremov, Artem-
Autor(es): dc.creatorGrishchuk, Ekaterina L.-
Autor(es): dc.creatorMcIntosh, J. Richard-
Autor(es): dc.creatorAtaullakhanov, Fazly I.-
Data de aceite: dc.date.accessioned2019-08-21T19:26:08Z-
Data de disponibilização: dc.date.available2019-08-21T19:26:08Z-
Data de envio: dc.date.issued2008-
Data de envio: dc.date.issued2009-06-09-
Data de envio: dc.date.issued2013-03-21-
Data de envio: dc.date.issued2013-03-21-
Data de envio: dc.date.issued2013-03-21-
Fonte completa do material: dc.identifierhttp://objetoseducacionais2.mec.gov.br/handle/mec/22989-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/491939-
Descrição: dc.descriptionThis video shows a shortening microtubules end 'Depolymerizing microtubule' and a ring coupler (red), which was modeled after the Dam1/DASH kinetochore complex from budding yeast. Each ring subunit binds to the middle of the outer surface of beta-tubulin with a flexible linker 4 nm in length (blue). This calculation shows a ring with 13 subunits, but the rings with more subunits are expected to behave similarly because the number of bonds that the ring can establish with the microtubule wall is determined by the symmetry of the microtubule lattice (13-fold). In this calculation, the energy of interaction between each linker and tubulin is low (3 kBT, where kB is Boltzmann constant) relative to the thermal energy, so the ring diffuses rapidly on the MT wall (the video stops when the ring moves beyond the microtubule segment that was used for this calculation). As the plus end of the microtubule disassembles, the ring's displacements become biased, i.e. they occur on average away from the shortening end and towards the bottom of the screen. This is because the ring motion in the opposite direction is interrupted by a mechanical barrier formed by the flared protofilaments. The energy necessary to straighten protofilaments is so high that the ring's thermal energy is not sufficient to pass this barrier. Although technically, the bending protofilaments push on the weakly bound ring when it comes in contact, this aspect of their interactions is mechanically insignificant, due to the low resistance of ring sliding. As a result, such a ring is expected to have no impact on the rate at which the microtubule shortens-
Descrição: dc.descriptionComponente Curricular::Educação Superior::Ciências Biológicas::Morfologia-
Idioma: dc.languagept_BR-
Publicador: dc.publisherThe American Society for Cell Biology, ASCB Image & Video Library-
Relação: dc.relationVideo 2. Ring video 2 - Coupler Moving via a Biased-Diffusion Mechanism.mov-
Direitos: dc.rightsYou may use the Images only for research and educational purposes in an educational institution. Images may be stored on a network server, hard drive or removable media, but only while this Agreement remains in effect. All of the Images you use must be accompanied by a notice stating, "© [Date and Author's or Owner's Name]. All rights reserved. Reprinted under license from The American Society for Cell Biology." The notice shall appear with the Images or on a credits page. "Educational purposes" means (i) non-commercial instruction or curriculum based teaching by educators to students at nonprofit educational institutions, (ii) planned non-commercial study, investigation or research directed toward making a contribution to a field of knowledge, or (iii) presentation of research findings at non-commercial peer conferences, workshops or seminars, but in any event there must be express written permission from ASCB before an Image can be published. "Educational institutions" include K-12 schools, colleges and universities. Libraries, museums, hospitals, research institutes and other nonprofit organizations are considered educational institutions when they engage in nonprofit instructional, research or scholarly activities for educational purposes-
???dc.source???: dc.sourcehttp://cellimages.ascb.org/cdm4/item_viewer.php?CISOROOT=/p4041coll12&CISOPTR=278&CISOBOX=1&REC=9-
Palavras-chave: dc.subjectDepolymerizing microtubule-
Palavras-chave: dc.subjectBeta-tubulin-
Palavras-chave: dc.subjectProtofilament-
Palavras-chave: dc.subjectEducação Superior::Ciências Biológicas::Morfologia::Citologia e Biologia Celular-
Título: dc.titleRing coupler moving via a biased-diffusion mechanism [Microtubule depolymerization as a biological machine]-
???dc.description2???: dc.description2To show a shortening microtubules end 'Depolymerizing microtubule' and a ring coupler (red), which was modeled after the Dam1/DASH kinetochore complex from budding yeast-
???dc.description3???: dc.description3Duration: 38 s. Contributors: E. Salova, A. Zheleznyakov, M. Molodtsov. This video was categorized by the site as: [Microtubule depolymerization as a biological machine: video 2]. Software required: Apple Quicktime 7 player. Available at: <http://www.apple.com/quicktime/download/win.html>-
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