A selection and analysis tool for fluorescence recovery after photobleaching (FRAP) region of interest (ROI)

Registro completo de metadados
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorStasevich, Timothy J.-
Data de aceite: dc.date.accessioned2019-08-21T18:25:05Z-
Data de disponibilização: dc.date.available2019-08-21T18:25:05Z-
Data de envio: dc.date.issued2016-10-26-
Data de envio: dc.date.issued2016-10-26-
Fonte completa do material: dc.identifierhttp://acervodigital.unesp.br/handle/unesp/365212-
Fonte completa do material: dc.identifierhttp://objetoseducacionais2.mec.gov.br/handle/mec/16128-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/471418-
Descrição: dc.descriptionFluorescence recovery after photobleaching (FRAP) can be used to quantify the mobility of molecules in live cells. In a typical FRAP experiment, a portion of a cell expressing a fluorescently tagged molecule of interest is photobleached and the recovery of the photobleached region is monitored as a function of time. Briefly, faster recoveries correspond to more mobile molecules. In this Demonstration a movie of a FRAP experiment is analyzed. You can scroll through the movie in the upper display and choose the size and position of a circular region of interest (ROI). The normalized intensity of the fluorescence in the ROI is displayed as a function of image number in a plot below. A vertical red line designates the current image number of the movie in the upper display-
Descrição: dc.descriptionComponente Curricular::Educação Superior::Multidisciplinar::Interdiciplinar-
Publicador: dc.publisherWolfram Demonstration Project-
Relação: dc.relationASelectionAndAnalysisToolForFluorescenceRecoveryAfterPhotobl.nbp-
Direitos: dc.rightsDemonstration freeware using MathematicaPlayer-
Palavras-chave: dc.subjectData analysis-
Palavras-chave: dc.subjectEducação Superior::Multidisciplinar::Ensino::Ensino de Ciências e Matemática-
Título: dc.titleA selection and analysis tool for fluorescence recovery after photobleaching (FRAP) region of interest (ROI)-
Tipo de arquivo: dc.typetexto-
Aparece nas coleções:Repositório Institucional - Acervo Digital Unesp

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