A structural in silico analysis of the immunogenicity of L-Asparaginase from Penicillium cerradense

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Brasília, School of Health Sciences, Department of Pharmacy, Laboratory of Natural Products-
Autor(es): dc.contributorUniversity of Brasília, School of Health Sciences, Department of Pharmacy, inSiliTox-
Autor(es): dc.contributorUniversity of Brasília, School of Health Sciences, Department of Pharmacy, inSiliTox-
Autor(es): dc.contributorUniversity of Brasília, School of Health Sciences, Department of Pharmacy, Laboratory of Natural Products-
Autor(es): dc.contributorUniversity of Brasília, School of Health Sciences, Department of Pharmacy, Laboratory of Natural Products-
Autor(es): dc.contributorUniversity of Brasília, School of Health Sciences, Department of Pharmacy, Laboratory of Natural Products-
Autor(es): dc.contributorUniversity of São Paulo, School of Pharmaceutical Sciences, Department of Biochemical and Pharmaceutical Technology-
Autor(es): dc.contributorUniversity of Brasilia, Institute Biological Sciences, Department Cell Biology-
Autor(es): dc.contributorUniversity of Brasília, School of Health Sciences, Department of Pharmacy, Laboratory of Natural Products-
Autor(es): dc.creatorAndrade, Kellen Cruvinel Rodrigues-
Autor(es): dc.creatorHomem de Mello, Mauricio-
Autor(es): dc.creatorMotta, Julia Almeida-
Autor(es): dc.creatorGuimarães, Marina Borges-
Autor(es): dc.creatorAbreu, Joel Antônio Cordeiro de-
Autor(es): dc.creatorSouza, Paula Monteiro de-
Autor(es): dc.creatorPessoa, Adalberto-
Autor(es): dc.creatorPappas Júnior, Georgios Joannis-
Autor(es): dc.creatorMagalhães, Pérola de Oliveira-
Data de aceite: dc.date.accessioned2026-08-11T11:01:51Z-
Data de disponibilização: dc.date.available2026-08-11T11:01:51Z-
Data de envio: dc.date.issued2025-10-27-
Data de envio: dc.date.issued2025-10-27-
Data de envio: dc.date.issued2023-
Fonte completa do material: dc.identifierhttp://repositorio.unb.br/handle/10482/52934-
Fonte completa do material: dc.identifierhttps://doi.org/10.3390/ijms25094788-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-4541-9177-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-8767-407X-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-5969-0806-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-5268-8690-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0002-1100-976X-
Fonte completa do material: dc.identifierhttps://orcid.org/0000-0001-8011-6940-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1186833-
Descrição: dc.descriptionL-asparaginase is an essential drug used to treat acute lymphoid leukemia (ALL), a cancer of high prevalence in children. Several adverse reactions associated with L-asparaginase have been observed, mainly caused by immunogenicity and allergenicity. Some strategies have been adopted, such as searching for new microorganisms that produce the enzyme and applying protein engineering. Therefore, this work aimed to elucidate the molecular structure and predict the immunogenic profile of L-asparaginase from Penicillium cerradense, recently revealed as a new fungus of the genus Penicillium and producer of the enzyme, as a motivation to search for alternatives to bacterial L-asparaginase. In the evolutionary relationship, L-asparaginase from P. cerradense closely matches Aspergillus species. Using in silico tools, we characterized the enzyme as a protein fragment of 378 amino acids (39 kDa), including a signal peptide containing 17 amino acids, and the isoelectric point at 5.13. The oligomeric state was predicted to be a homotetramer. Also, this L-asparaginase presented a similar immunogenicity response (T- and B-cell epitopes) compared to Escherichia coli and Dickeya chrysanthemi enzymes. These results suggest a potentially useful L-asparaginase, with insights that can drive strategies to improve enzyme production.-
Descrição: dc.descriptionFaculdade de Ciências da Saúde (FS)-
Descrição: dc.descriptionDepartamento de Farmácia (FS FAR)-
Descrição: dc.descriptionInstituto de Ciências Biológicas (IB)-
Descrição: dc.descriptionDepartamento de Biologia Celular (IB CEL)-
Descrição: dc.descriptionPrograma de Pós-Graduação em Ciências Farmacêuticas-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Publicador: dc.publisherMDPI-
Direitos: dc.rightsAcesso Aberto-
Palavras-chave: dc.subjectL-asparaginase-
Palavras-chave: dc.subjectImunogenicidade-
Palavras-chave: dc.subjectPenicillium cerradense-
Título: dc.titleA structural in silico analysis of the immunogenicity of L-Asparaginase from Penicillium cerradense-
Aparece nas coleções:Repositório Institucional – UNB

Não existem arquivos associados a este item.