Shoot-tip cryopreservation by droplet vitrification of Byrsonima intermedia A. Juss.: a woody tropical and medicinal plant species from Brazilian Cerrado

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorSilva, Luciano Coutinho-
Autor(es): dc.creatorPaiva, Renato-
Autor(es): dc.creatorSwennen, Rony-
Autor(es): dc.creatorAndrè, Edwige-
Autor(es): dc.creatorPanis, Bart-
Data de aceite: dc.date.accessioned2026-02-09T12:55:00Z-
Data de disponibilização: dc.date.available2026-02-09T12:55:00Z-
Data de envio: dc.date.issued2020-02-10-
Data de envio: dc.date.issued2020-02-10-
Data de envio: dc.date.issued2013-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/38993-
Fonte completa do material: dc.identifierhttp://www.cryoletters.org/Abstracts/vol_34_4_2013.htm#338-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1170851-
Descrição: dc.descriptionCryopreservation of plant species is poorly investigated in Brazil. The aim of this study was to cryopreserve Byrsonima intermedia shoot apical meristems through droplet vitrification. A culture medium for shoot-tips growth was established using the Woody Plant Medium supplemented with 2.22 µM 6-benzylaminopurine. Excised shoot-tips were subjected to pre-culture and/or post-culture treatments on Murashige and Skoog medium with 0.3 M sucrose for 24 h prior dehydration on PVS2 at 0°C for 15, 30 or 45 minutes prior to plunging in liquid nitrogen. The effect of 15 days of shoot pre-growth on a high osmotic medium (0.3 M sucrose or 0.21 M sorbitol + 0.09 M sucrose) prior to meristem excision and cryopreservation was also investigated. Pre-culturing shoot-tips on 0.3 M sucrose for 24 h prior to cryopreservation increased the regrowth level after thawing to 90%. Shoot-tips excised from shoots pre-grown on MS + 0.21 M sorbitol + 0.09 M sucrose for 15 days presented a satisfactory regrowth level (67%).-
Idioma: dc.languageen-
Publicador: dc.publisherCryoLetters LLP-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceCryo-Letters-
Palavras-chave: dc.subjectPre-culture effect-
Palavras-chave: dc.subjectPost-culture effect-
Palavras-chave: dc.subjectPre-growth effect-
Palavras-chave: dc.subjectSorbitol-
Palavras-chave: dc.subjectMedicinal plant-
Palavras-chave: dc.subjectVitrification-
Título: dc.titleShoot-tip cryopreservation by droplet vitrification of Byrsonima intermedia A. Juss.: a woody tropical and medicinal plant species from Brazilian Cerrado-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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