Motility and fertility of the subtropical freshwater fish streaked prochilod (Prochilodus lineatus) sperm cryopreserved in powdered coconut water

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorViveiros, Ana Tereza de Mendonça-
Autor(es): dc.creatorNascimento, Ariane Flávia do-
Autor(es): dc.creatorOrfão, Laura Helena-
Autor(es): dc.creatorIsaú, Ziara Aparecida-
Data de aceite: dc.date.accessioned2026-02-09T12:53:49Z-
Data de disponibilização: dc.date.available2026-02-09T12:53:49Z-
Data de envio: dc.date.issued2016-12-15-
Data de envio: dc.date.issued2016-12-15-
Data de envio: dc.date.issued2010-09-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br//handle/1/12077-
Fonte completa do material: dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0093691X10001780-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1170436-
Descrição: dc.descriptionStreaked prochilod (Prochilodus lineatus) is a freshwater fish inhabiting many South American rivers. The objective was to determine the effectiveness of coconut water (ACP™), combined with methylglycol, as a freezing medium for streaked prochilod sperm. A secondary objective was to compare a computer-assisted sperm analyzer (CASA) system versus subjective microscropic examination as a means of assessing sperm motility. As a control, glucose and methylglycol was used, according to our previous study. Sperm diluted in each medium was loaded into 0.5 mL straws, frozen in liquid nitrogen vapor (in a dry shipper), and stored in liquid nitrogen (-196 °C). Half of the samples were evaluated for sperm motility, both subjectively and with CASA; the remainder were evaluated for fertility. There was no difference (P > 0.05) between subjective or CASA assessment of post-thaw sperm motility. Although sperm motility was higher in sperm cryopreserved in ACP™ (85%) than in glucose (75%), cryopreservation in either extender yielded similar fertilization rates (46–48%) and sperm velocities. There were positive correlations (r = 0.56–0.8) between all sperm velocities and fertilization rate. In conclusion, streaked prochilod sperm cryopreserved in glucose or ACP™ and methylglycol was fertile, and thus could be used for research or commercial settings. Furthermore, although the CASA system provided objective data regarding sperm motility, in the present study, subjective evaluation of sperm motility was practical and a good indication of sperm quality; it could readily be done by well-trained personnel under field or laboratory conditions.-
Idioma: dc.languageen-
Publicador: dc.publisherElsevier-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceTheriogenology-
Palavras-chave: dc.subjectProchilodus lineatus-
Palavras-chave: dc.subjectSemen cryopreservation-
Palavras-chave: dc.subjectSperm motility-
Palavras-chave: dc.subjectFertility-
Palavras-chave: dc.subjectFish-
Título: dc.titleMotility and fertility of the subtropical freshwater fish streaked prochilod (Prochilodus lineatus) sperm cryopreserved in powdered coconut water-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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