In vitro conservation and cryopreservation of Nandina domestica, an Outdoor Ornamental Shrub

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorOzudogru, Aylin-
Autor(es): dc.creatorSilva, Diogo Pedrosa Corrêa da-
Autor(es): dc.creatorKaya, Ergun-
Autor(es): dc.creatorDradi, Giuliano-
Autor(es): dc.creatorPaiva, Renato-
Autor(es): dc.creatorLambardi, Maurizio-
Data de aceite: dc.date.accessioned2026-02-09T12:52:41Z-
Data de disponibilização: dc.date.available2026-02-09T12:52:41Z-
Data de envio: dc.date.issued2020-02-10-
Data de envio: dc.date.issued2020-02-10-
Data de envio: dc.date.issued2013-12-06-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/38994-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1170042-
Descrição: dc.descriptionThe study focused on an economically-important ornamental outdoor shrub, Nandina domestica, with the aims to (i) optimize an effective in vitro conservation method, and (ii) develop a cryopreservation protocol for shoot tips by the PVS2 vitrification and droplet-vitrification techniques. For in vitro conservation of shoot cultures, the tested parameters were sucrose content in the storage medium (30, 45, 60 g/L) and storage temperature (4 °C or 8 °C). Cryopreservation was performed by applying the PVS2 vitrification solution, in 2-ml cryovials or in drops over aluminum foil strips, for 15, 30, 60 or 90 min at 0 °C, followed by the direct immersion in liquid nitrogen of shoot tips. Results show that N. domestica shoots can be conserved successfully for 6 months at both the temperatures tested, especially when 60 g/L sucrose is used in the storage medium. However, conservation at 4 °C showed to be more appropriate, as hyperhydricity was observed in post-conservation of shoots coming from storage at 8 °C. As for cryopreservation, a daily gradual increase of sucrose concentration (from 0.25 to 1.0 M) produced better protection to the samples that were stored in liquid nitrogen. Indeed, with this sucrose treatment method, a 30-min PVS2 incubation time was enough to produce, 60 days after thawing, the best recovery (47% and 50%) of shoot tips, cryopreserved with PVS2 vitrification and droplet-vitrification, respectively.-
Formato: dc.formatapplication/pdf-
Idioma: dc.languageen-
Publicador: dc.publisherAcademicPres Publishing House (EAP)-
Direitos: dc.rightsacesso aberto-
Direitos: dc.rightshttp://creativecommons.org/licenses/by/4.0/-
Direitos: dc.rightshttp://creativecommons.org/licenses/by/4.0/-
???dc.source???: dc.sourceNotulae Botanicae Horti Agrobotanici Cluj-Napoca-
Palavras-chave: dc.subjectCold storage-
Palavras-chave: dc.subjectDroplet-vitrification-
Palavras-chave: dc.subjectLiquid nitrogen-
Palavras-chave: dc.subjectOne-step freezing-
Palavras-chave: dc.subjectPVS2-vitrification-
Palavras-chave: dc.subjectSlow growth storage-
Título: dc.titleIn vitro conservation and cryopreservation of Nandina domestica, an Outdoor Ornamental Shrub-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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