Impact of multi-metals (Cd, Pb and Zn) exposure on the physiology of the yeast Pichia kudriavzevii

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.creatorMesquita, Vanessa A.-
Autor(es): dc.creatorMachado, Manuela D.-
Autor(es): dc.creatorSilva, Cristina F.-
Autor(es): dc.creatorSoares, Eduardo V.-
Data de aceite: dc.date.accessioned2026-02-09T12:48:18Z-
Data de disponibilização: dc.date.available2026-02-09T12:48:18Z-
Data de envio: dc.date.issued2017-08-09-
Data de envio: dc.date.issued2017-08-09-
Data de envio: dc.date.issued2015-07-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br/handle/1/15099-
Fonte completa do material: dc.identifierhttps://link.springer.com/article/10.1007%2Fs11356-015-4326-9-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1168609-
Descrição: dc.descriptionMetal contamination of the environment is frequently associated to the presence of two or more metals. This work aimed to study the impact of a mixture of metals (Cd, Pb and Zn) on the physiology of the non-conventional yeast Pichia kudriavzevii. The incubation of yeast cells with 5 mg/l Cd, 10 mg/l Pb and 5 mg/l Zn, for 6 h, induced a loss of metabolic activity (assessed by FUN-1 staining) and proliferation capacity (evaluated by a clonogenic assay), with a small loss of membrane integrity (measured by trypan blue exclusion assay). The staining of yeast cells with calcofluor white revealed that no modification of chitin deposition pattern occurred during the exposure to metal mixture. Extending for 24 h, the exposure of yeast cells to metal mixture provoked a loss of membrane integrity, which was accompanied by the leakage of intracellular components. A marked loss of the metabolic activity and the loss of proliferation capacity were also observed. The analysis of the impact of a single metal has shown that, under the conditions studied, Pb was the metal responsible for the toxic effect observed in the metal mixture. Intracellular accumulation of Pb seems to be correlated with the metals’ toxic effects observed.-
Idioma: dc.languageen-
Publicador: dc.publisherSpringer-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceEnvironmental Science and Pollution Research-
Palavras-chave: dc.subjectCell dead-
Palavras-chave: dc.subjectMetal contamination-
Palavras-chave: dc.subjectMetabolic activity inhibition-
Palavras-chave: dc.subjectMetals toxicity-
Palavras-chave: dc.subjectYeast survival-
Palavras-chave: dc.subjectMorte celular-
Palavras-chave: dc.subjectContaminação por metais-
Palavras-chave: dc.subjectInibição da atividade metabólica-
Palavras-chave: dc.subjectToxicidade nos metais-
Palavras-chave: dc.subjectSobrevida de fermento-
Título: dc.titleImpact of multi-metals (Cd, Pb and Zn) exposure on the physiology of the yeast Pichia kudriavzevii-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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