Effects of cooling rates on the quality of Prochilodus lineatus (Valenciennes, 1836) sperm

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MetadadosDescriçãoIdioma
Autor(es): dc.creatorPaula, Daniella Aparecida-
Autor(es): dc.creatorMurgas, Luis David Solis-
Autor(es): dc.creatorCastro, Tássia Flávia Dias-
Autor(es): dc.creatorAssis, Isadora-
Autor(es): dc.creatorReis Neto, Rafael Vilhena-
Autor(es): dc.creatorMarcussi, Silvana-
Data de aceite: dc.date.accessioned2026-02-09T12:47:30Z-
Data de disponibilização: dc.date.available2026-02-09T12:47:30Z-
Data de envio: dc.date.issued2020-05-18-
Data de envio: dc.date.issued2020-05-18-
Data de envio: dc.date.issued2019-07-
Fonte completa do material: dc.identifierhttps://repositorio.ufla.br//handle/1/41030-
Fonte completa do material: dc.identifierhttps://onlinelibrary.wiley.com/doi/10.1111/rda.13459-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/capes/1168360-
Descrição: dc.descriptionThis study aims to investigate the effect of different cooling rates on the semen cryopreservation of curimba (Prochilodus lineatus). Nineteen ejaculates were obtained from adults males and cryopreserved at 15°C/min (CR15), 30°C/min (CR30) (controlled temperature inside and outside straw, speed was stable during freezing) and direct freezing in liquid nitrogen vapour (~35.6°C/min) (CRNV). The straws were thawed and seminal parameters evaluated. DNA fragmentation through the comet assay was assessed. A fresh sperm sample was not frozen and used for analyses. Data were submitted to an analysis of variance (ANOVA), and means were compared by Scott–Knott test (p < 0.05) using the R Software. Mean motility percentage was 100%, and motility duration was 39.5 ± 5.7 s for the fresh sperm (subjective analysis); 58.9 ± 8.0% and 24.5 ± 5.7 s for CR15; 64.8 ± 4.8% and 26.5 ± 7.1 s for CR30; and 50.1 ± 16% and 25.7 ± 4.7 s for CRNV, respectively. Motility percentages were higher and equal between CR15 and CR30 compared to CRNV (p < 0.05). Some sperm motion kinetics, namely average path velocity (VAP) and straight line velocity (VAS), were higher for CR30 (p < 0.05), while curvilinear velocity (VCL) and velocity progression (PRO) were lower for CRNV (p < 0.05). Straightness (STR) and wobble (WOB) were the same among treatments (p > 0.05). Sperm morphology results indicated higher means for total morphological sperm alterations in CRNV. All cooling rates caused sperm DNA fragmentation, although CR30 provided a less harmful effect. This is the first report for cryopreserved P. lineatus sperm preserved under different controlled cooling rates. The cooling rate of 30°C/min is indicated for the cryopreservation of this fish sperm as it led to the lowest detrimental spermatozoa effects.-
Idioma: dc.languageen-
Direitos: dc.rightsrestrictAccess-
???dc.source???: dc.sourceReproduction in Domestic Animals-
Palavras-chave: dc.subjectCryopreservation-
Palavras-chave: dc.subjectFreezing rate-
Palavras-chave: dc.subjectNeotropical fish-
Palavras-chave: dc.subjectReproduction-
Título: dc.titleEffects of cooling rates on the quality of Prochilodus lineatus (Valenciennes, 1836) sperm-
Tipo de arquivo: dc.typeArtigo-
Aparece nas coleções:Repositório Institucional da Universidade Federal de Lavras (RIUFLA)

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